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The ePetri dish, an on-chip cell imaging platform based on subpixel perspective sweeping microscopy (SPSM)

Zheng, Guoan and Lee, Seung Ah and Antebi, Yaron and Elowitz, Michael B. and Yang, Changhuei (2011) The ePetri dish, an on-chip cell imaging platform based on subpixel perspective sweeping microscopy (SPSM). Proceedings of the National Academy of Sciences of the United States of America, 108 (41). pp. 16889-16894. ISSN 0027-8424. PMCID PMC3193234.

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We report a chip-scale lensless wide-field-of-view microscopy imaging technique, subpixel perspective sweeping microscopy, which can render microscopy images of growing or confluent cell cultures autonomously. We demonstrate that this technology can be used to build smart Petri dish platforms, termed ePetri, for cell culture experiments. This technique leverages the recent broad and cheap availability of high performance image sensor chips to provide a low-cost and automated microscopy solution. Unlike the two major classes of lensless microscopy methods, optofluidic microscopy and digital in-line holography microscopy, this new approach is fully capable of working with cell cultures or any samples in which cells may be contiguously connected. With our prototype, we demonstrate the ability to image samples of area 6 mm × 4 mm at 660-nm resolution. As a further demonstration, we showed that the method can be applied to image color stained cell culture sample and to image and track cell culture growth directly within an incubator. Finally, we showed that this method can track embryonic stem cell differentiations over the entire sensor surface. Smart Petri dish based on this technology can significantly streamline and improve cell culture experiments by cutting down on human labor and contamination risks.

Item Type:Article
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URLURL TypeDescription DOIArticle CentralArticle
Elowitz, Michael B.0000-0002-1221-0967
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2011 National Academy of Sciences. Freely available online through the PNAS open access option. Edited by David A. Weitz, Harvard University, Cambridge, MA, and approved August 23, 2011 (received for review July 5, 2011). Published online before print October 3, 2011. We thank Dr. Benjamin Judkewitz for HeLa cell tracking and Mr. Samuel Yang for analyzing some of the data. We acknowledge funding support from the Coulter Foundation. Author contributions: G.Z. and C.Y. designed research; G.Z., S.A.L., and Y.A. performed research; G.Z. analyzed data; and G.Z., S.A.L., Y.A., M.B.E., and C.Y. wrote the paper.
Funding AgencyGrant Number
Wallace H. Coulter FoundationUNSPECIFIED
Subject Keywords:lensless imaging; time-lapse microscopy; on-chip cellular imaging; stem cell differentiation tracking; superresolution algorithm
Issue or Number:41
PubMed Central ID:PMC3193234
Record Number:CaltechAUTHORS:20111108-160027564
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:27685
Deposited By: Jason Perez
Deposited On:09 Nov 2011 00:19
Last Modified:09 Mar 2020 13:19

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