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Thermodynamic basis for the genome to capsid charge relationship in viral encapsidation

Ting, Christina L. and Wu, Jianzhong and Wang, Zhen-Gang (2011) Thermodynamic basis for the genome to capsid charge relationship in viral encapsidation. Proceedings of the National Academy of Sciences of the United States of America, 108 (41). pp. 16986-16991. ISSN 0027-8424. PMCID PMC3193252. https://resolver.caltech.edu/CaltechAUTHORS:20111109-073727841

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Abstract

We establish an appropriate thermodynamic framework for determining the optimal genome length in electrostatically driven viral encapsidation. Importantly, our analysis includes the electrostatic potential due to the Donnan equilibrium, which arises from the semipermeable nature of the viral capsid, i.e., permeable to small mobile ions but impermeable to charged macromolecules. Because most macromolecules in the cellular milieu are negatively charged, the Donnan potential provides an additional driving force for genome encapsidation. In contrast to previous theoretical studies, we find that the optimal genome length is the result of combined effects from the electrostatic interactions of all charged species, the excluded volume and, to a very significant degree, the Donnan potential. In particular, the Donnan potential is essential for obtaining negatively overcharged viruses. The prevalence of overcharged viruses in nature may suggest an evolutionary preference for viruses to increase the amount of genome packaged by utilizing the Donnan potential (through increases in the capsid radius), rather than high charges on the capsid, so that structural stability of the capsid is maintained.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.1109307108DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc3193252/PatentArticle
ORCID:
AuthorORCID
Wu, Jianzhong0000-0002-4582-5941
Wang, Zhen-Gang0000-0002-3361-6114
Additional Information:© 2011 by the National Academy of Sciences. Edited by David A. Tirrell, California Institute of Technology, Pasadena, CA, and approved August 26, 2011 (received for review June 9, 2011). Published online before print October 3, 2011. Z.-G.W. and C.L.T. acknowledge support from the Jacobs Institute for Molecular Engineering for Medicine at Caltech. C.L.T. is thankful for financial support from an National Institute of Health (NIH)training grant. Author contributions: J.W. and Z.-G.W. designed research; C.L.T. and Z.-G.W. performed research; C.L.T. and Z.-G.W. analyzed data; and C.L.T. and Z.-G.W. wrote the paper. The authors declare no conflict of interest. This Direct Submission article had a prearranged editor.
Group:Jacobs Institute for Molecular Engineering for Medicine
Funders:
Funding AgencyGrant Number
Joseph J. Jacobs Institute for Molecular Engineering for MedicineUNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Subject Keywords:polyelectrolyte; viral assembly
Issue or Number:41
PubMed Central ID:PMC3193252
Record Number:CaltechAUTHORS:20111109-073727841
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20111109-073727841
Official Citation: Christina L. Ting, Jianzhong Wu, and Zhen-Gang Wang Thermodynamic basis for the genome to capsid charge relationship in viral encapsidation PNAS 2011 108 (41) 16986-16991; published ahead of print October 3, 2011, doi:10.1073/pnas.1109307108
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:27690
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:09 Nov 2011 15:52
Last Modified:02 Jun 2020 20:49

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