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Regulation of CNS and motor axon guidance in Drosophila by the receptor tyrosine phosphatase DPTP52F

Schindelholz, Benno and Knirr, Matthias and Warrior, Rahul and Zinn, Kai (2001) Regulation of CNS and motor axon guidance in Drosophila by the receptor tyrosine phosphatase DPTP52F. Development, 128 (21). pp. 4371-4382. ISSN 0950-1991.

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Receptor-linked protein tyrosine phosphatases (RPTPs) regulate axon guidance and synaptogenesis in Drosophila embryos and larvae. We describe DPTP52F, the sixth RPTP to be discovered in Drosophila. Our genomic analysis indicates that there are likely to be no additional RPTPs encoded in the fly genome. Five of the six Drosophila RPTPs have C. elegans counterparts, and three of the six are also orthologous to human RPTP subfamilies. DPTP52F, however, has no clear orthologs in other organisms. The DPTP52F extracellular domain contains five fibronectin type III repeats and it has a single phosphatase domain. DPTP52F is selectively expressed in the CNS of late embryos, as are DPTP10D, DLAR, DPTP69D and DPTP99A. To define developmental roles of DPTP52F, we used RNA interference (RNAi)-induced phenotypes as a guide to identify Ptp52F alleles among a collection of EMS-induced lethal mutations. Ptp52F single mutant embryos have axon guidance phenotypes that affect CNS longitudinal tracts. This phenotype is suppressed in Dlar Ptp52F double mutants, indicating that DPTP52F and DLAR interact competitively in regulating CNS axon guidance decisions. Ptp52F single mutations also cause motor axon phenotypes that selectively affect the SNa nerve. DPTP52F, DPTP10D and DPTP69D have partially redundant roles in regulation of guidance decisions made by axons within the ISN and ISNb motor nerves.

Item Type:Article
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Zinn, Kai0000-0002-6706-5605
Additional Information:© 2001 The Company of Biologists Limited. Accepted 10 August 2001. We thank Lakshmi Bugga for performing the RT-PCR analysis of the 5' end of Ptp52F mRNA, Peter Snow (Caltech Protein Expression Facility) for expression of DPTP52F fusion proteins, Susan Ou (Caltech Monoclonal Antibody Facility) for generation of anti DPTP52F mAbs, J. Duncan and Y. Lei for help in generation and complementation analysis of the EMS alleles, Aloisia Schmid for discussions about Ptp52F phenotypes, neuroblast lineages and RNAi, and other members of the Zinn group for discussions and comments on the manuscript. B. S. was supported by postdoctoral fellowships from Caltech and from the Swiss National Science Foundation. This work was supported by NIH RO1 grant NS28182 to K. Z, and by a basic research grant from the March of Dimes Birth Defects Foundation to R. W.
Funding AgencyGrant Number
NIHRO1 NS28182
March of Dimes Birth Defects FoundationUNSPECIFIED
Swiss National Science Foundation (SNSF)UNSPECIFIED
Subject Keywords:Receptor tyrosine phosphatase, PTP, Drosophila, Neural development, Axon guidance, Neuromuscular system, Genomics, Motor axon, RNAi
Issue or Number:21
Record Number:CaltechAUTHORS:20111118-154151997
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Official Citation:Benno Schindelholz, Matthias Knirr, Rahul Warrior, and Kai Zinn Regulation of CNS and motor axon guidance in Drosophila by the receptor tyrosine phosphatase DPTP52F Development 2001 128:4371-4382
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:27874
Deposited By: Ruth Sustaita
Deposited On:21 Nov 2011 17:48
Last Modified:03 Oct 2019 03:27

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