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Microscopy refocusing and dark-field imaging by using a simple LED array

Zheng, Guoan and Kolner, Christopher and Yang, Changhuei (2011) Microscopy refocusing and dark-field imaging by using a simple LED array. Optics Letters, 36 (20). pp. 3987-3989. ISSN 0146-9592.

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The condenser is one of the main components in most transmitted light compound microscopes. In this Letter, we show that such a condenser can be replaced by a programmable LED array to achieve greater imaging flexibility and functionality. Without mechanically scanning the sample or changing the microscope setup, the proposed approach can be used for dark-field imaging, bright-field imaging, microscopy sectioning, and digital refocusing. Images of a starfish embryo were acquired by using such an approach for demonstration.

Item Type:Article
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Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2011 Optical Society of America. Received July 14, 2011; revised September 9, 2011; accepted September 12, 2011; posted September 13, 2011 (Doc. ID 151079); published October 5, 2011. We acknowledge funding support from United States Department of Defense under grant W81XWH-09-1-0051.
Funding AgencyGrant Number
Department of DefenseW81XWH-09-1-0051
Issue or Number:20
Classification Code:OCIS codes: 110.0180, 110.2945, 110.3010, 180.6900.
Record Number:CaltechAUTHORS:20111129-093810678
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Official Citation:Guoan Zheng, Christopher Kolner, and Changhuei Yang, "Microscopy refocusing and dark-field imaging by using a simple LED array," Opt. Lett. 36, 3987-3989 (2011)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:27985
Deposited By: Ruth Sustaita
Deposited On:29 Nov 2011 17:48
Last Modified:09 Mar 2020 13:19

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