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Distinct and Redundant Functions of µ1 Medium Chains of the AP-1 Clathrin-Associated Protein Complex in the Nematode Caenorhabditis elegans

Shim, Jaegal and Sternberg, Paul W. and Lee, Junho (2000) Distinct and Redundant Functions of µ1 Medium Chains of the AP-1 Clathrin-Associated Protein Complex in the Nematode Caenorhabditis elegans. Molecular Biology of the Cell, 11 (8). pp. 2743-2756. ISSN 1059-1524. PMCID PMC14953. http://resolver.caltech.edu/CaltechAUTHORS:20111209-094319607

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Abstract

In the nematode Caenorhabditis elegans, there exist two μ1 medium chains of the AP-1 clathrin-associated protein complex. Mutations of unc-101, the gene that encodes one of the μ1 chains, cause pleiotropic effects (Lee et al., 1994 blue right-pointing triangle). In this report, we identified and analyzed the second μ1 chain gene, apm-1. Unlike the mammalian homologs, the two medium chains are expressed ubiquitously throughout development. RNA interference (RNAi) experiments with apm-1 showed that apm-1 and unc-101 were redundant in embryogenesis and in vulval development. Consistent with this, a hybrid protein containing APM-1, when overexpressed, rescued the phenotype of an unc-101 mutant. However, single disruptions of apm-1 or unc-101 have distinct phenotypes, indicating that the two medium chains may have distinct functions. RNAi of any one of the small or large chains of AP-1 complex (σ1, β1, or γ) showed a phenotype identical to that caused by the simultaneous disruption of unc-101 and apm-1, but not that by single disruption of either gene. This suggests that the two medium chains may share large and small chains in the AP-1 complexes. Thus, apm-1 and unc-101 encode two highly related μ1 chains that share redundant and distinct functions within AP-1 clathrin-associated protein complexes of the same tissue.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.molbiolcell.org/content/11/8/2743.abstractPublisherArticle
http://dx.doi.org/10.1091/mbc.11.8.2743DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC14953/PubMed CentralArticle
ORCID:
AuthorORCID
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2000 by The American Society for Cell Biology. Under the License and Publishing Agreement, authors grant to the general public, effective two months after publication of (i.e.,. the appearance of) the edited manuscript in an online issue of MBoC, the nonexclusive right to copy, distribute, or display the manuscript subject to the terms of the Creative Commons–Noncommercial–Share Alike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0). Submitted May 12, 2000; Revised May 15, 2000; Accepted June 2, 2000. Monitoring Editor: Juan Bonifacino. We thank Drs. A. Fire, A. Coulson, and Y. Kohara, and the Caenorhabditis Genetics Center (St. Paul, MN) for providing the nematode vectors, strains, and genomic and cDNA clones. We also thank S. Yoo and Dr. D. Jeoung for the dendrogram of the medium chain homologs. This study was supported in part by the Korean /U.S. Cooperative Science Program (KOSEF 965-0504-001–2), by the Molecular Medicine Research Group Program (98-J03– 01-01-A-05) from the Ministry of Science and Technology (Korea) to J.L., and by USPHS grant HD23690 to P.W.S. P.W.S. is an investigator with the H. H. M. I.
Funders:
Funding AgencyGrant Number
Korean/U.S. Cooperative Science ProgramKOSEF 965-0504-001-2
Molecular Medicine Research Group Program98-J03-01-01-A-05
Ministry of Science and Technology (Korea)UNSPECIFIED
USPHSHD23690
PubMed Central ID:PMC14953
Record Number:CaltechAUTHORS:20111209-094319607
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20111209-094319607
Official Citation:Jaegal Shim, Paul W. Sternberg, and Junho Lee Distinct and Redundant Functions of μ1 Medium Chains of the AP-1 Clathrin-Associated Protein Complex in the NematodeCaenorhabditis elegans
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:28388
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:09 Dec 2011 18:23
Last Modified:18 Jul 2017 14:32

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