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Patterning of the C. elegans 1° vulval lineage by RAS and Wnt pathways

Wang, Minqin and Sternberg, Paul W. (2000) Patterning of the C. elegans 1° vulval lineage by RAS and Wnt pathways. Development, 127 (23). pp. 5047-5058. ISSN 0950-1991.

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In C. elegans, the descendants of the 1° vulval precursor cell (VPC) establish a fixed spatial pattern of two different cell fates: E-F-F-E. The two inner granddaughters attach to the somatic gonadal anchor cell (AC) and generate four vulF cells, while the two outer granddaughters produce four vulE progeny. zmp-1::GFP, a molecular marker that distinguishes these two fates, is expressed in vulE cells, but not vulF cells. We demonstrate that a short-range AC signal is required to ensure that the pattern of vulE and vulF fates is properly established. In addition, signaling between the inner and outer 1° VPC descendants, as well as intrinsic polarity of the 1° VPC daughters, is involved in the asymmetric divisions of the 1° VPC daughters and the proper orientation of the outcome. Finally, we provide evidence that RAS signaling is used during this new AC signaling event, while the Wnt receptor LIN-17 appears to mediate signaling between the inner and outer 1° VPC descendants.

Item Type:Article
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Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2000 The Company of Biologists. Accepted 8 September; published on WWW 2 November 2000. We are grateful to Jim Butler and Jim Kramer for zmp-1::GFP DNA, Carola Sigrist and Ralf Sommer for Pristionchus hs-ras(dn) DNA, and Bhagwati Gupta and Martha Kirouac for integrating syEx282. We thank Tom Clandinin, Dave Sherwood, Nadeem Moghal, Takao Inoue, Bhagwati Gupta, Byung Joon Hwang, and Lisa Girard for critically reading the manuscript. Some of the strains were provided by the C. elegans Genetics Center. This work was supported by USPHS (grant HD23690) to P.W.S., an Investigator with the Howard Hughes Medical Institute.
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:Pattern formation, 1° lineage, Execution, Asymmetric cell division, C. elegans
Issue or Number:23
Record Number:CaltechAUTHORS:20111209-102248415
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Official Citation: M. Wang and P.W. Sternberg Patterning of the C. elegans 1 degrees vulval lineage by RAS and Wnt pathways Development 2000 127:5047-5058
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:28394
Deposited By: Ruth Sustaita
Deposited On:09 Dec 2011 19:23
Last Modified:03 Oct 2019 03:31

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