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Live imaging of endogenous periodic tryptophan protein 2 gene homologue during zebrafish development

Jayasena, Chathurani S. and Trinh, Le A. and Bronner, Marianne E. (2011) Live imaging of endogenous periodic tryptophan protein 2 gene homologue during zebrafish development. Developmental Dynamics, 240 (11). pp. 2578-2583. ISSN 1058-8388. https://resolver.caltech.edu/CaltechAUTHORS:20111216-162340043

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Abstract

Yeast Periodic tryptophan protein 2 gene (Pwp2) is involved in ribosome biogenesis and has been implicated in regulation of the cell cycle in yeast. Here, we report a zebrafish protein-trap line that produces fluorescently tagged Periodic tryptophan protein 2 gene homologue (Pwp2h) protein, which can be dynamically tracked in living fish at subcellular resolution. We identified both full-length zebrafish Pwp2h and a short variant. The expression results show that Pwp2h is present in numerous sites in the early developing embryo, but later is restricted to highly proliferative regions, including the forebrain ventricular zone and endoderm-derived organs in the early larval stage. At the subcellular level, Pwp2h protein appears to be localized to the region of the nucleolus consistent with its presumed function in ribosomal RNA synthesis. This Pwp2h protein trap line offers a powerful tool to study the link between ribosome biogenesis and cell cycle progression during vertebrate development.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/dvdy.22744 DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/dvdy.22744/abstractPublisherArticle
ORCID:
AuthorORCID
Bronner, Marianne E.0000-0003-4274-1862
Additional Information:© 2011 Wiley Periodicals, Inc. Accepted 30 August 2011. Published online 26 September 2011. We thank Ho-Yin Leung for performing 30 RACE and LeighAnn Fletcher for technical help in the Caltech Centers of Excellence in Genomic Science fish facility. This work is supported by USPHS P50HG004071.
Funders:
Funding AgencyGrant Number
NIHP50HG004071
Subject Keywords:Pwp2h; zebrafish; ribosome biogenesis; development
Issue or Number:11
Record Number:CaltechAUTHORS:20111216-162340043
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20111216-162340043
Official Citation:Jayasena, C. S., Trinh, L. A. and Bronner, M. (2011), Live imaging of endogenous periodic tryptophan protein 2 gene homologue during zebrafish development. Developmental Dynamics, 240: 2578–2583. doi: 10.1002/dvdy.22744
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:28503
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 Dec 2011 18:28
Last Modified:03 Oct 2019 03:33

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