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Activated chemoreceptor arrays remain intact and hexagonally packed

Briegel, Ariane and Beeby, Morgan and Thanbichler, Martin and Jensen, Grant J. (2011) Activated chemoreceptor arrays remain intact and hexagonally packed. Molecular Microbiology, 82 (3). pp. 748-757. ISSN 0950-382X. PMCID PMC3641884. https://resolver.caltech.edu/CaltechAUTHORS:20111219-115630715

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Abstract

Bacterial chemoreceptors cluster into exquisitively sensitive, tunable, highly ordered, polar arrays. While these arrays serve as paradigms of cell signalling in general, it remains unclear what conformational changes transduce signals from the periplasmic tips, where attractants and repellents bind, to the cytoplasmic signalling domains. Conflicting reports support and contest the hypothesis that activation causes large changes in the packing arrangement of the arrays, up to and including their complete disassembly. Using electron cryotomography, here we show that in Caulobacter crescentus, chemoreceptor arrays in cells grown in different media and immediately after exposure to the attractant galactose all exhibit the same 12 nm hexagonal packing arrangement, array size and other structural parameters. ΔcheB and ΔcheR mutants mimicking attractant- or repellent-bound states prior to adaptation also show the same lattice structure. We conclude that signal transduction and amplification must be accomplished through only small, nanoscale conformational changes.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1111/j.1365-2958.2011.07854.xDOIArticle
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2011.07854.x/abstractPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641884/PubMed CentralArticle
ORCID:
AuthorORCID
Briegel, Ariane0000-0003-3733-3725
Beeby, Morgan0000-0001-6413-9835
Thanbichler, Martin0000-0002-1303-1442
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2011 Blackwell Publishing Ltd. Accepted 14 September, 2011. Article first published online: 12 Oct 2011. This work was supported in part by the Howard Hughes Medical Institute and by gifts to Caltech from the Gordon and Betty Moore Foundation.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Issue or Number:3
PubMed Central ID:PMC3641884
Record Number:CaltechAUTHORS:20111219-115630715
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20111219-115630715
Official Citation:Briegel, A., Beeby, M., Thanbichler, M. and Jensen, G. J. (2011), Activated chemoreceptor arrays remain intact and hexagonally packed. Molecular Microbiology, 82: 748–757. doi: 10.1111/j.1365-2958.2011.07854.x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:28518
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:20 Dec 2011 17:47
Last Modified:03 Oct 2019 03:33

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