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Transducing methyltransferase activity into electrical signals in a carbon nanotube–DNA device

Wang, Hanfei and Muren, Natalie B. and Ordinario, David and Gorodetsky, Alon A. and Barton, Jacqueline K. and Nuckolls, Colin (2012) Transducing methyltransferase activity into electrical signals in a carbon nanotube–DNA device. Chemical Science, 3 (1). pp. 62-65. ISSN 2041-6520. PMCID PMC3399246. https://resolver.caltech.edu/CaltechAUTHORS:20120104-142531511

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Abstract

This study creates a device where the DNA is electronically integrated to serve as both the biological target and electrical transducer in a CNT–DNA–CNT device. We detect DNA binding and methylation by the methyltransferase M.SssI at the single molecule level. We demonstrate sequence-specific, reversible binding of M.SssI and protein-catalyzed methylation that alters the protein-binding affinity of the device. This device, which relies on the exquisite electrical sensitivity of DNA, represents a unique route for the specific, single molecule detection of enzymatic activity.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1039/c1sc00772f DOIArticle
http://pubs.rsc.org/en/Content/ArticleLanding/2012/SC/c1sc00772fPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3399246/PubMed CentralArticle
ORCID:
AuthorORCID
Gorodetsky, Alon A.0000-0002-3811-552X
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2012 The Royal Society of Chemistry. Received 07 Oct 2011, Accepted 19 Oct 2011. First published on the web 20 Oct 2011. We are grateful to the National Institute of Health (GM61077 to JKB), the Office of Naval Research (N00014-09-1-1117 to JKB and CN), the National Science Foundation NIRT (ECCS-07-07748 to CN), and the National Science Foundation (CHE0936923 to AAG) for their financial support.
Funders:
Funding AgencyGrant Number
NIHGM61077
Office of Naval Research (ONR)N00014-09-1-1117
NSFECCS-07-07748
NSFCHE0936923
Issue or Number:1
PubMed Central ID:PMC3399246
Record Number:CaltechAUTHORS:20120104-142531511
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120104-142531511
Official Citation:Transducing methyltransferase activity into electrical signals in a carbon nanotube–DNA device Hanfei Wang, Natalie B. Muren, David Ordinario, Alon A. Gorodetsky, Jacqueline K. Barton and Colin Nuckolls Chem. Sci., 2012, 3, 62-65 DOI: 10.1039/C1SC00772F
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:28655
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:04 Jan 2012 22:47
Last Modified:03 Oct 2019 03:34

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