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Reproductive Meristem22 is a unique marker for the early stages of stamen development

Romanel, Elisson and Das, Pradeep and Amasino, Richard M. and Traas, Jan and Meyerowitz, Elliot and Alves-Ferreira, Marcio (2011) Reproductive Meristem22 is a unique marker for the early stages of stamen development. International Journal of Developmental Biology, 55 (6). pp. 657-664. ISSN 0214-6282. doi:10.1387/ijdb.113340er.

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Stamens undergo a very elaborate development program that gives rise not only to many specific tissue types, but also to the male gametes. The specification of stamen identity is coordinated by a group of homeotic genes such as APETALA3 (AP3) and PISTILLATA (PI), AGAMOUS (AG) and SEPALLATA (SEP1-4) genes. Genome-wide transcriptomic comparisons between floral buds of wild-type and ap3 mutants led to the identification of the REM22 gene, which is expressed in the early stages of stamen development. This gene is member of the plant-specific B3 DNA-binding superfamily. In this work, we dissect the spatio-temporal expression pattern of REM22 during the early stages of stamen development. To this end, both in situ hybridization analyses as well as in vivo fluorescence strategies were employed. At stage 4 of flower development, REM22 is expressed exclusively in those undifferentiated cells of the floral meristem that will give rise to the stamen primordia. At stage 5, REM22 expression is restricted to the epidermal and the subepidermal layers of anther primordia. Later, this expression is confined to the middle layer and the differentiating tapetal cells. After stage 10 when all the tissues of the anther have differentiated, REM22 expression is no longer detectable. Furthermore, we examined the pREM22::GUS-GFP marker line in an inducible system where the ectopic AG function is used to promote microsporogenesis. The data support the idea that REM22 expression is a useful marker to study the early stages of stamen development.

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Meyerowitz, Elliot0000-0003-4798-5153
Additional Information:© 2011 UBC Press. Accepted: 27 July 2011. Final, author-corrected PDF published online: 24 August 2011. Edited by: Mieke van Lijsebettens. E.R. thanks Annick Dubois and Pradeep Das for friendship and hosting assistance to develop this work; to Robert Franks for comments on the manuscript; and to Marcus Heisler for initial analysis of REM22 marker line by confocal microscopy. M.A.-F. was supported by grants from CNPq and Fundação de Amparo à Pesquisa do Rio de Janeiro (FAPERJ; M. Alves-Ferreira: #E-26/102.861/2008). This work is part of E.R.`PhD thesis from Department of Genetics of the Universidade Federal do Rio de Janeiro, Brazil. E.R. was supported by a PhD studentship (Conselho Nacional de Desenvolvimento Cientifico e Technolόgico (CNPq) and UNESCO.
Funding AgencyGrant Number
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNSPECIFIED
Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)E-26/102.861/2008
Subject Keywords:male sporogenesis, B3 domain, reproductive meristem, stamen development, REM22 function
Issue or Number:6
Record Number:CaltechAUTHORS:20120106-075814440
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Official Citation:Reproductive Meristem22 is a unique marker for the early stages of stamen development Elisson Romanel, Pradeep Das, Richard M. Amasino, Jan Traas, Elliot Meyerowitz and Marcio Alves-Ferreira Int. J. Dev. Biol. (2011) 55: 657-664
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:28685
Deposited By: Ruth Sustaita
Deposited On:06 Jan 2012 16:28
Last Modified:09 Nov 2021 16:59

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