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Coordinate actions of BMPs, Wnts, Shh and noggin mediate patterning of the dorsal somite

Marcelle, Christophe and Stark, Michael R. and Bronner-Fraser, Marianne (1997) Coordinate actions of BMPs, Wnts, Shh and noggin mediate patterning of the dorsal somite. Development, 124 (20). pp. 3955-3963. ISSN 0950-1991.

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Shortly after their formation, somites of vertebrate embryos differentiate along the dorsoventral axis into sclerotome, myotome and dermomyotome. The dermomyotome is then patterned along its mediolateral axis into medial, central and lateral compartments, which contain progenitors of epaxial muscle, dermis and hypaxial muscle, respectively. Here, we used Wnt-11 as a molecular marker for the medial compartment of dermomyotome (the ‘medial lip’) to demonstrate that BMP in the dorsal neural tube indirectly induces formation of the medial lip by up-regulating Wnt-1 and Wnt-3a (but not Wnt-4) expression in the neural tube. Noggin in the dorsal somite may inhibit the direct action of BMP on this tissue. Wnt-11 induction is antagonized by Sonic Hedgehog, secreted by the notochord and the floor plate. Together, our results show that the coordinated actions of the dorsal neural tube (via BMP and Wnts), the ventral neural tube/notochord (via Shh) and the somite itself (via noggin) mediates patterning of the dorsal compartment of the somite.

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Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1997 The Company of Biologists Limited. Accepted 1 August 1997. We thank Drs Charles Ordahl and Scott Fraser for critical reading of the manuscript and Johnny Choi and Sung Hee Kil for their help during this research. We are grateful to the following for their generous gifts of reagents used in this study: Dr Paul Brickell for the chick BMP-4 probe used for in situ hybridization and the mouse BMP-4 cDNA cloned in the RCAS(A) retroviral vector, Dr Richard Harland for the noggin-secreting CHO cells, Dr Cliff Tabin for the chick Sonic hedgehog cDNA cloned in the RCAS(A) retroviral vector and for the chick Patched probe, Dr Bruce Patterson for the chick MyoD probe, Dr Anthony Brown for the Wnt-1-expressing cell line and Dr Jonathan Cooke for the chick noggin probe. This work was supported by grants from the Muscular Dystrophy Foundation to C. M. and M. B. F.
Funding AgencyGrant Number
Muscular Dystrophy FoundationUNSPECIFIED
Subject Keywords:somite; dermomyotome; BMP; Sonic Hedgehog; Wnt; noggin; chick
Issue or Number:20
Record Number:CaltechAUTHORS:20120119-093000980
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:28847
Deposited By: Tony Diaz
Deposited On:19 Jan 2012 19:20
Last Modified:03 Oct 2019 03:36

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