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Prp43: An RNA helicase-like factor involved in spliceosome disassembly

Arenas, Jaime E. and Abelson, John N. (1997) Prp43: An RNA helicase-like factor involved in spliceosome disassembly. Proceedings of the National Academy of Sciences of the United States of America, 94 (22). pp. 11798-11802. ISSN 0027-8424. PMCID PMC23592. doi:10.1073/pnas.94.22.11798.

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The Saccharomyces cerevisiae genes PRP2, PRP16, and PRP22 encode pre-mRNA splicing factors that belong to the highly conserved “DEAH” family of putative RNA helicases. We previously identified two additional members of this family, JA1 and JA2. To investigate its biological function, we cloned the JA1 gene and generated alleles carrying mutations identical to those found in highly conserved regions of other members of the DEAH family. A ja1 allele carrying a mutation identical to that in the temperature-sensitive (ts) prp22–1 gene conferred ts phenotype when integrated into the genome of a wild-type strain by gene replacement. Northern analysis of RNA obtained from the ts strain shifted to a nonpermissive temperature revealed accumulation of unspliced pre-mRNAs and excised intron lariats. Furthermore, analysis of splicing complexes showed that intron lariats accumulated in spliceosomes. The results presented indicate that JA1 encodes a pre-mRNA processing factor (Prp) involved in disassembly of spliceosomes after the release of mature mRNA. We have therefore renamed this gene PRP43.

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Additional Information:© 1997 by The National Academy of Sciences. Contributed by John N. Abelson, August 8, 1997. We thank P. Raghunathan and C. Guthrie for sharing results prior to publication, Yan Wang for review of the manuscript, and J. Messenger for sequencing of the JA2 gene. This work was supported by National Institutes of Health Grant GM32637. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked ‘‘advertisement’’ in accordance with 18 U.S.C. §1734 solely to indicate this fact.
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Issue or Number:22
PubMed Central ID:PMC23592
Record Number:CaltechAUTHORS:20120119-110410201
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Official Citation:Jaime E. Arenas and John N. Abelson Prp43: An RNA helicase-like factor involved in spliceosome disassembly PNAS 1997 94 (22) 11798-11802
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:28857
Deposited By: Ruth Sustaita
Deposited On:19 Jan 2012 19:53
Last Modified:09 Nov 2021 17:01

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