CaltechAUTHORS
  A Caltech Library Service

Expression patterns of mRNAs for methanotrophy and thiotrophy in symbionts of the hydrothermal vent mussel Bathymodiolus puteoserpentis

Wendeberg, Annelie and Zielinski, Frank U. and Borowski, Christian and Dubilier, Nicole (2012) Expression patterns of mRNAs for methanotrophy and thiotrophy in symbionts of the hydrothermal vent mussel Bathymodiolus puteoserpentis. ISME Journal, 6 (1). pp. 104-112. ISSN 1751-7362. PMCID PMC3246237. doi:10.1038/ismej.2011.81. https://resolver.caltech.edu/CaltechAUTHORS:20120131-094048509

[img]
Preview
PDF (Figure S1) - Supplemental Material
See Usage Policy.

4MB
[img]
Preview
PDF (Figure S2) - Supplemental Material
See Usage Policy.

13MB
[img]
Preview
PDF (Figure S3) - Supplemental Material
See Usage Policy.

4MB
[img] MS Word - Supplemental Material
See Usage Policy.

45kB

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20120131-094048509

Abstract

The hydrothermal vent mussel Bathymodiolus puteoserpentis (Mytilidae) from the Mid-Atlantic Ridge hosts symbiotic sulfur- and methane-oxidizing bacteria in its gills. In this study, we investigated the activity and distribution of these two symbionts in juvenile mussels from the Logatchev hydrothermal vent field (14°45′N Mid-Atlantic Ridge). Expression patterns of two key genes for chemosynthesis were examined: pmoA (encoding subunit A of the particulate methane monooxygenase) as an indicator for methanotrophy, and aprA (encoding the subunit A of the dissimilatory adenosine-5′-phosphosulfate reductase) as an indicator for thiotrophy. Using simultaneous fluorescence in situ hybridization (FISH) of rRNA and mRNA we observed highest mRNA FISH signals toward the ciliated epithelium where seawater enters the gills. The levels of mRNA expression differed between individual specimens collected in a single grab from the same sampling site, whereas no obvious differences in symbiont abundance or distribution were observed. We propose that the symbionts respond to the steep temporal and spatial gradients in methane, reduced sulfur compounds and oxygen by modifying gene transcription, whereas changes in symbiont abundance and distribution take much longer than regulation of mRNA expression and may only occur in response to long-term changes in vent fluid geochemistry.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/ismej.2011.81 DOIArticle
http://www.nature.com/ismej/journal/v6/n1/full/ismej201181a.htmlPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246237/PubMed CentralArticle
ORCID:
AuthorORCID
Dubilier, Nicole0000-0002-9394-825X
Additional Information:© 2012 International Society for Microbial Ecology. Received 11 February 2011; revised 10 May 2011; accepted 17 May 2011; published online 7 July 2011. We are grateful to Silke Wetzel for excellent technical assistance, and Rudi Amann and Shana Goffredi for critical reading of the manuscript and valuable discussions. We thank the chief scientist Thomas Kuhn and the captain of the Hydromar I cruise as well as the crews of the RV Meteor and the ROV Quest (MARUM, University of Bremen, Germany). This work was supported by the Priority Program 1144 ‘From Mantle to Ocean: Energy-, Material- and Life-cycles at Spreading Axes’ (Contribution number 58) of the German Science Foundation (DFG), the DFG-Research Center/Excellence Cluster ‘The Ocean in the Earth System’ at MARUM, and the Max Planck Society.
Funders:
Funding AgencyGrant Number
Deutsche Forschungsgemeinschaft (DFG)1144
Max Planck SocietyUNSPECIFIED
Subject Keywords:symbiosis; Bathymodiolus; mRNA FISH
Issue or Number:1
PubMed Central ID:PMC3246237
DOI:10.1038/ismej.2011.81
Record Number:CaltechAUTHORS:20120131-094048509
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120131-094048509
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29035
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Mar 2012 21:00
Last Modified:09 Nov 2021 17:03

Repository Staff Only: item control page