Silverman, Scott K. and Lester, Henry A. and Dougherty, Dennis A. (1996) Subunit Stoichiometry of a Heteromultimeric G protein-coupled Inward-rectifier K^+ Channel. Journal of Biological Chemistry, 271 (48). pp. 30524-30528. ISSN 0021-9258. doi:10.1074/jbc.271.48.30524. https://resolver.caltech.edu/CaltechAUTHORS:20120207-144820693
![[img]](https://authors.library.caltech.edu/style/images/fileicons/application_pdf.png)
|
PDF
- Published Version
See Usage Policy. 427kB |
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20120207-144820693
Abstract
We investigated the stoichiometry of the heteromultimeric G protein-coupled inward-recitfier K^+ channel (GIRK) formed from GIRK1 and GIRK4 subunits. Multimeric GIRK constructs with several concatenated channel subunits were expressed in Xenopus oocytes. Coexpression of various trimeric constructs with different monomers clearly showed that the functional channel has stoichiometry (GIRK1)_2(GIRK4)_2. Efforts to establish a preferred arrangement of subunits around the channel pore suggest that more than one arrangement may be viable.
| Item Type: | Article | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Related URLs: |
| ||||||||||
| ORCID: |
| ||||||||||
| Additional Information: | © 1996 by The American Society for Biochemistry and Molecular Biology, Inc. Received June 5, 1996. Revision received July 23, 1996. We thank Paulo Kofuji for many helpful discussions. This work was supported by National Institutes of Health Grants MH49176, GM29836, NS11756, and NS34407. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. | ||||||||||
| Funders: |
| ||||||||||
| Issue or Number: | 48 | ||||||||||
| DOI: | 10.1074/jbc.271.48.30524 | ||||||||||
| Record Number: | CaltechAUTHORS:20120207-144820693 | ||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20120207-144820693 | ||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
| ID Code: | 29176 | ||||||||||
| Collection: | CaltechAUTHORS | ||||||||||
| Deposited By: | Tony Diaz | ||||||||||
| Deposited On: | 21 Mar 2012 20:53 | ||||||||||
| Last Modified: | 09 Nov 2021 17:04 |
Repository Staff Only: item control page
