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Polydactylous limbs in Strong's Luxoid mice result from ectopic polarizing activity

Chan, David C. and Laufer, Ed and Tabin, Cliff and Leder, Philip (1995) Polydactylous limbs in Strong's Luxoid mice result from ectopic polarizing activity. Development, 121 (7). pp. 1971-1978. ISSN 0950-1991.

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Strong's Luxoid (1st^D) is a semidominant mouse mutation in which heterozygotes show preaxial hindlimb polydactyly, and homozygotes show fore- and hindlimb polydactyly. The digit patterns of these polydactylous limbs resemble those caused by polarizing grafts, since additional digits with posterior character are present at the anterior side of the limb. Such observations suggest that 1st^D limb buds might contain a genetically determined ectopic region of polarizing activity. Accordingly, we show that mutant embryos ectopically express the pattern-determining genes fibroblast growth factor 4 (fgf-4), sonic hedgehog (shh), and Hoxd-12 in the anterior region of the limb. Further, we show that anterior mesoderm from mutant limbs exhibits polarizing activity when grafted into host chicken limbs. In contrast to an experimentally derived polydactylous transgenic mouse, forelimbs of homozygotes show a normal pattern of Hoxb-8 expression, indicating that the duplication of polarizing tissue here occurs downstream or independently of Hoxb-8. We suggest that the 1st gene product is involved in anteroposterior axis formation during normal limb development.

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Chan, David C.0000-0002-0191-2154
Additional Information:© 1995 The Company of Biologists Limited. Accepted 21 March 1995. We are grateful to Anne Burke, Andrew McMahon, and Gail Martin for providing probes. We thank members of the Leder and Tabin labs for helpful advice and comments.
Subject Keywords:Strong’s Luxoid, limb mutant, limb development, polarizing activity, apical ectodermal ridge, mouse
Issue or Number:7
Record Number:CaltechAUTHORS:20120208-075531654
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29185
Deposited By: Tony Diaz
Deposited On:21 Mar 2012 20:49
Last Modified:24 Feb 2020 10:30

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