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Rational design and tuning of ribozyme-based devices

Liang, Joe C. and Smolke, Christina D. (2012) Rational design and tuning of ribozyme-based devices. In: Ribozymes: Methods and Protocols. Methods in Molecular Biology. No.848. Humana Press , pp. 439-454. ISBN 9781617795442.

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A synthetic gene-regulatory device platform was described by modularly assembling three RNA components encoding distinct functions of sensing, transmission, and actuation. The molecular binding at the sensor component is translated by the transmitter component through a strand-displacement event to modulate activity of the actuator component, which then interacts with cellular transcriptional machinery to affect gene expression levels. Here, we provide some general guidelines on linking RNA components to construct gene-regulatory devices and strategies to tune device regulatory activities through an RNA folding program for specific cellular applications.

Item Type:Book Section
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URLURL TypeDescription
Smolke, Christina D.0000-0002-5449-8495
Additional Information:© 2012 Springer Science+Business Media, LLC. The Smolke Laboratory is supported by funds from the NIH, NSF, and the Alfred P. Sloan Foundation.
Funding AgencyGrant Number
Alfred P. Sloan FoundationUNSPECIFIED
Subject Keywords:Rational design, Ribozyme, RNA folding, RNA switch, Synthetic biology
Series Name:Methods in Molecular Biology
Issue or Number:848
Record Number:CaltechAUTHORS:20120213-081043360
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29251
Deposited By: Tony Diaz
Deposited On:14 Feb 2012 00:26
Last Modified:03 Oct 2019 03:39

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