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Segmental migration of trunk neural crest: time-lapse analysis reveals a role for PNA-binding molecules

Krull, Catherine E. and Collazo, Andres and Fraser, Scott E. and Bronner-Fraser, Marianne (1995) Segmental migration of trunk neural crest: time-lapse analysis reveals a role for PNA-binding molecules. Development, 121 (11). pp. 3733-3743. ISSN 0950-1991.

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Trunk neural crest cells migrate through the somites in a striking segmental fashion, entering the rostral but not caudal sclerotome, via cues intrinsic to the somites. Attempts to define the molecular bases of these cues have been hampered by the lack of an accessible assay system. To examine trunk neural crest migration over time and to perturb candidate guiding molecules, we have developed a novel explant preparation. Here, we demonstrate that trunk regions of the chicken embryo, placed in explant culture, continue to develop apparently normally for 2 days. Neural crest cells, recognized by prelabeling with DiI or by poststaining with the HNK-1 antibody, migrate in the somites of the explants in their typical segmental pattern. Furthermore, this paradigm allows us to follow trunk neural crest migration in situ for the first time using low-light-level videomicroscopy. The trajectories of individual neural crest cells were often complex, with cells migrating in an episodic mode encompassing forward, backward and lateral movements. Frequently, neural crest cells migrated in close-knit groups of 2–4 cells, moving at mean rates of migration of 10–14 µm/hour. Treatment of the explants with the lectin peanut agglutinin (PNA) both slowed the rate and altered the pattern of neural crest migration. Neural crest cells entered both the rostral and caudal halves of the sclerotome with mean rates of migration ranging from 6 to 13 µm/hour. These results suggest that peanut agglutinin-binding molecules are required for the segmental patterning of trunk neural crest migration. Because this approach permits neural crest migration to be both observed and perturbed, it offers the promise of more direct assays of the factors that influence neural crest development.

Item Type:Article
Related URLs:
URLURL TypeDescription
Fraser, Scott E.0000-0002-5377-0223
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1995 The Company of Biologists Limited. Accepted 14 August 1995. We dedicate this work to the memory of Dr Edmund Arbas. His great enthusiasm for his family, friends and science will be remembered. We thank Roham Zamanian and Jon Neri for excellent technical assistance. This work was supported by NIH/NRSA 09459 to C. E. K., by USPHS 15527 and a Muscular Dystrophy Association grant to M. B.-F., by a NIMH Silvio Conte Center grant to S. E. F. and A. C., and Beckman Institute Biological Imaging Center.
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)15527
Muscular Dystrophy AssociationUNSPECIFIED
National Institute of Mental Health (NIMH)UNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Subject Keywords:somite, explant culture, videomicroscopy, avian, neural crest, PNA
Issue or Number:11
Record Number:CaltechAUTHORS:20120215-092512792
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29297
Deposited By: Tony Diaz
Deposited On:21 Mar 2012 16:58
Last Modified:03 Oct 2019 03:40

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