Chistoserdova, Ludmila V. and Lidstrom, Mary E. (1994) Genetics of the serine cycle in Methylobacterium extorquens AM1: identification of sgaA and mtdA and sequences of sgaA, hprA, and mtdA. Journal of Bacteriology, 176 (7). pp. 1957-1968. ISSN 0021-9193. PMCID PMC205300. https://resolver.caltech.edu/CaltechAUTHORS:20120221-104435221
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Abstract
In a previous paper, we reported identification of the 5' part of hprA of Methylobacterium extorquens AM1, which encodes the serine cycle enzyme hydroxypyruvate reductase (L. V. Chistoserdova and M. E. Lidstrom, J. Bacteriol. 174:71-77, 1992). Here we present the complete sequence of hprA and partial sequence of genes adjacent to hprA. Upstream of hprA, the 3' part of an open reading frame was discovered, separated from hprA by 263 bp. This open reading frame was identified as the gene encoding another serine cycle enzyme, serine glyoxylate aminotransferase (sgaA). Cells containing an insertion mutation into sgaA were unable to grow on C1 compounds, demonstrating that the gene is required for C1 metabolism. Sequencing downstream of hprA has revealed the presence of another open reading frame (mtdA), which is probably cotranscribed with hprA. This open reading frame was identified as the gene required for the synthesis of 5,10-methylenetetrahydrofolate dehydrogenase. Our data suggest that this enzyme plays an integral role in methylotrophic metabolism in M. extorquens AM1, either in formaldehyde oxidation or as part of the serine cycle.
Item Type: | Article | |||||||||
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Additional Information: | © 1994 American Society for Microbiology. Received 13 December 1993; Accepted 17 January 1994. This work was supported by NIH grant GM36294. | |||||||||
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Issue or Number: | 7 | |||||||||
PubMed Central ID: | PMC205300 | |||||||||
Record Number: | CaltechAUTHORS:20120221-104435221 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20120221-104435221 | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 29388 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | Tony Diaz | |||||||||
Deposited On: | 22 Feb 2012 00:35 | |||||||||
Last Modified: | 03 Oct 2019 03:41 |
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