Bell, Christina and Smith, Geoffrey T. and Sweredoski, Michael J. and Hess, Sonja (2012) Characterization of the Mycobacterium tuberculosis Proteome by Liquid Chromatography Mass Spectrometry-based Proteomics Techniques: A Comprehensive Resource for Tuberculosis Research. Journal of Proteome Research, 11 (1). pp. 119-130. ISSN 1535-3893. doi:10.1021/pr2007939. https://resolver.caltech.edu/CaltechAUTHORS:20120222-093637862
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Abstract
Approximately, one-third of the world’s population is infected with Mycobacterium tuberculosis, the causative agent of Tuberculosis. Secreted and membrane proteins that interact with the host play important roles for the pathogenicity of the bacteria and are potential drug targets or components of vaccines. In this present study, subcellular fractionation in combination with membrane enrichment was used to comprehensively analyze the M. tuberculosis proteome. The proteome of the M. tuberculosis cell wall, membrane, cytosol, lysate, and culture filtrate was defined with a high coverage. Exceptional enrichment for membrane proteins was achieved using wheat germ agglutinin (WGA)-affinity two-phase partitioning, a technique that has to date not yet been exploited for the enrichment of mycobacterial membranes. Overall, 1051 M. tuberculosis protein groups including 183 transmembrane proteins have been identified by LC–MS/MS analysis using stringent database search criteria with a minimum of two peptides and an estimated FDR of less than 1%. With many mycobacterial antigens and lipoglycoproteins identified, the results from this study suggest that many of the newly discovered proteins could represent potential candidates mediating host–pathogen interactions. In addition, this data set provides experimental information about protein localization and thus serves as a valuable resource for M. tuberculosis proteome research.
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Additional Information: | © 2011 American Chemical Society. Received: August 17, 2011. Publication Date (Web): November 7, 2011. This work was supported by the Betty and Gordon Moore Foundation and the Beckman Institute. We thank Colorado State University, Denver, CO (http//www.cvmbs.colostate.edu/mip/tb/) and the Biodefense and Emerging Infections Research Resource Repository (Manassas, VA) for providing M. tuberculosis H37Rv fractions. | |||||||||
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Subject Keywords: | Mycobacterium tuberculosis; membrane proteins; WGA; enrichment; protein localization; mass spectrometry | |||||||||
Issue or Number: | 1 | |||||||||
DOI: | 10.1021/pr2007939 | |||||||||
Record Number: | CaltechAUTHORS:20120222-093637862 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20120222-093637862 | |||||||||
Official Citation: | Characterization of the Mycobacterium tuberculosis Proteome by Liquid Chromatography Mass Spectrometry-based Proteomics Techniques: A Comprehensive Resource for Tuberculosis Research Christina Bell, Geoffrey T. Smith, Michael J. Sweredoski, and Sonja Hess Journal of Proteome Research 2012 11 (1), 119-130 | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 29409 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | Ruth Sustaita | |||||||||
Deposited On: | 22 Feb 2012 18:10 | |||||||||
Last Modified: | 09 Nov 2021 17:06 |
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