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Molecular mechanisms of neural crest cell attachment and migration on types I and IV collagen

Perris, Roberto and Syfrig, Josef and Paulsson, Mats and Bronner-Fraser, Marianne (1993) Molecular mechanisms of neural crest cell attachment and migration on types I and IV collagen. Journal of Cell Science, 106 (4). pp. 1357-1368. ISSN 0021-9533.

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We have examined the mechanisms involved in the interaction of avian neural crest cells with collagen types I and IV (Col I and IV) during their adhesion and migration in vitro. For this purpose native Col IV was purified from chicken tissues, characterized biochemically and ultrastructurally. Purified chicken Col I and Col IV, and various proteolytic fragments of the collagens, were used in quantitative cell attachment and migration assays in conjunction with domain-specific collagen antibodies and antibodies to avian integrin subunits. Neural crest cells do not distinguish between different macromolecular arrangements of Col I during their initial attachment, but do so during their migration, showing a clear preference for polymeric Col I. Interaction with Col I is mediated by the α1β1 integrin, through binding to a segment of the α1(I) chain composed of fragment CNBr3. Neural crest cell attachment and migration on Col IV involves recognition of conformation-dependent sites within the triple-helical region and the noncollagenous, carboxyl-terminal NC1 domain. This recognition requires integrity of inter- and intrachain disulfide linkages and correct folding of the molecule. Moreover, there also is evidence that interaction sites within the NC1 domain may be cryptic, being exposed during migration of the cells in the intact collagen as a result of the prolonged cell-substratum contact. In contrast to Col I, neural crest cell interaction with Col IV is mediated by β1-class integrins other than α1β1.

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Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1993 The Company of Biologists Limited. Received 23 July 1993 - Accepted 14 September 1993. We are grateful to Drs Klaus Kühn, Jörgen Wieslander, Helene Sage, Charles Little, Saryu Dixit and John Harding for providing various purified intact and fragmented collagens, and anti-collagen antibodies. Tammy Dillon, Susan Tran and Kristie Bruk-Artinger are thanked for their invaluable technical assistance. The work was supported from grants from NIH (to M.B.-F.) and from the Swiss National Science Foundation (to M.P.).
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Swiss National Science Foundation (SNSF)UNSPECIFIED
Subject Keywords:neural crest cells, migration, collagen, adhesion
Record Number:CaltechAUTHORS:20120301-105050290
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29547
Deposited By: Tony Diaz
Deposited On:20 Mar 2012 22:22
Last Modified:02 Jan 2016 02:27

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