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In vivo volumetric imaging of human retinal circulation with phase-variance optical coherence tomography

Kim, Dae Yu and Fingler, Jeff and Werner, John S. and Schwartz, Daniel M. and Fraser, Scott E. and Zawadzki, Robert J. (2011) In vivo volumetric imaging of human retinal circulation with phase-variance optical coherence tomography. Biomedical Optics Express, 2 (6). pp. 1504-1513. ISSN 2156-7085. PMCID PMC3114219.

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We present in vivo volumetric images of human retinal micro-circulation using Fourier-domain optical coherence tomography (Fd-OCT) with the phase-variance based motion contrast method. Currently fundus fluorescein angiography (FA) is the standard technique in clinical settings for visualizing blood circulation of the retina. High contrast imaging of retinal vasculature is achieved by injection of a fluorescein dye into the systemic circulation. We previously reported phase-variance optical coherence tomography (pvOCT) as an alternative and non-invasive technique to image human retinal capillaries. In contrast to FA, pvOCT allows not only noninvasive visualization of a two-dimensional retinal perfusion map but also volumetric morphology of retinal microvasculature with high sensitivity. In this paper we report high-speed acquisition at 125 kHz A-scans with pvOCT to reduce motion artifacts and increase the scanning area when compared with previous reports. Two scanning schemes with different sampling densities and scanning areas are evaluated to find optimal parameters for high acquisition speed in vivo imaging. In order to evaluate this technique, we compare pvOCT capillary imaging at 3x3 mm^2 and 1.5x1.5 mm^2 with fundus FA for a normal human subject. Additionally, a volumetric view of retinal capillaries and a stitched image acquired with ten 3x3 mm^2 pvOCT sub-volumes are presented. Visualization of retinal vasculature with pvOCT has potential for diagnosis of retinal vascular diseases.

Item Type:Article
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URLURL TypeDescription CentralArticle
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2011 Optical Society of America. Received 14 Mar 2011; revised 9 May 2011; accepted 27 Apr 2011; published 11 May 2011. The authors thank Suman Pilli, Susan Garcia from the Vision Science and Advanced Retinal Imaging Laboratory, Department of Ophthalmology & Vision Science at the UC Davis Medical Center for help with OCT data acquisition. This research was supported by the Howard Hughes Medical Institute (HHMI) Med-into-Grad Initiative (HHMI-MIG 56006769), National Eye Institute (EY 014743), Research to Prevent Blindness (RPB), Beckman Institute, and That Man May See Foundation.
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)HHMI-MIG 56006769
National Eye InstituteEY 014743
Research to Prevent BlindnessUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
That Man May See FoundationUNSPECIFIED
Issue or Number:6
Classification Code:OCIS codes: 110.4500; 120.3890; 170.0110; 170.4470
PubMed Central ID:PMC3114219
Record Number:CaltechAUTHORS:20120306-100747214
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Official Citation:Dae Yu Kim, Jeff Fingler, John S. Werner, Daniel M. Schwartz, Scott E. Fraser, and Robert J. Zawadzki, "In vivo volumetric imaging of human retinal circulation with phase-variance optical coherence tomography," Biomed. Opt. Express 2, 1504-1513 (2011)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29588
Deposited By: Ruth Sustaita
Deposited On:13 Mar 2012 21:48
Last Modified:03 Oct 2019 03:42

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