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Visualizing Neuromodulation In Vivo: TANGO-Mapping of Dopamine Signaling Reveals Appetite Control of Sugar Sensing

Inagaki, Hidehiko K. and Ben-Tabou de-Leon, Shlomo and Wong, Allan M. and Jagadish, Smitha and Ishimoto, Hiroshi and Barnea, Gilad and Kitamoto, Toshihiro and Axel, Richard and Anderson, David J. (2012) Visualizing Neuromodulation In Vivo: TANGO-Mapping of Dopamine Signaling Reveals Appetite Control of Sugar Sensing. Cell, 148 (3). pp. 583-595. ISSN 0092-8674. PMCID PMC3295637. doi:10.1016/j.cell.2011.12.022.

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Behavior cannot be predicted from a "connectome" because the brain contains a chemical ‘‘map’’ of neuromodulation superimposed upon its synaptic connectivity map. Neuromodulation changes how neural circuits process information in different states, such as hunger or arousal. Here we describe a genetically based method to map, in an unbiased and brain-wide manner, sites of neuromodulation under different conditions in the Drosophila brain. This method, and genetic perturbations, reveal that the well-known effect of hunger to enhance behavioral sensitivity to sugar is mediated, at least in part, by the release of dopamine onto primary gustatory sensory neurons, which enhances sugar-evoked calcium influx. These data reinforce the concept that sensory neurons constitute an important locus for state-dependent gain control of behavior and introduce a methodology that can be extended to other neuromodulators and model organisms.

Item Type:Article
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URLURL TypeDescription CentralArticle
Wong, Allan M.0000-0002-8492-2162
Anderson, David J.0000-0001-6175-3872
Additional Information:© 2012 Elsevier Inc. Under an Elsevier user license. Received: July 14, 2011; Revised: October 7, 2011; Accepted: December 23, 2011; Published: February 2, 2012. We thank Dr. K.J. Lee for sharing Tango DNA constructs prior to publication. We also thank Drs. T. Lee, K. Deisseroth, A. Stathopoulos, and B. Pfeiffer for plasmids. Fly stocks were generously provided by the Bloomington Stock Center, the VDRC stock center, the Drosophila RNAi Screening Center, and Drs. G.M. Rubin, J. Simpson, L.L Looger, H. Keshishian, K. Scott, T. Lee, S. Birman, P.A. Garrity, C.Q. Doe, and K. Ito. We also thank Drs. H. Otsuna and Y. Wan for Fluorender and members of the Anderson lab for helpful discussion and sharing of flies. H.K.I. is supported by the Nakajima Foundation. G.B. is a Pew scholar and is supported in part by NIH grant 5R01MH086920. D.J.A. is an investigator of the Howard Hughes Medical Institute. This work was supported in part by NIH grant 1RO1 DA031389 to D.J.A.
Funding AgencyGrant Number
Nakajima FoundationUNSPECIFIED
NIH1RO1 DA031389
Issue or Number:3
PubMed Central ID:PMC3295637
Record Number:CaltechAUTHORS:20120312-110130340
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Official Citation:Hidehiko K. Inagaki, Shlomo Ben-Tabou de-Leon, Allan M. Wong, Smitha Jagadish, Hiroshi Ishimoto, Gilad Barnea, Toshihiro Kitamoto, Richard Axel, David J. Anderson, Visualizing Neuromodulation In Vivo: TANGO-Mapping of Dopamine Signaling Reveals Appetite Control of Sugar Sensing, Cell, Volume 148, Issue 3, 3 February 2012, Pages 583-595, ISSN 0092-8674, 10.1016/j.cell.2011.12.022.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29690
Deposited By: Jason Perez
Deposited On:12 Mar 2012 22:53
Last Modified:09 Nov 2021 19:28

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