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Cell motility driving mediolateral intercalation in explants of Xenopus laevis

Shih, John and Keller, Ray (1992) Cell motility driving mediolateral intercalation in explants of Xenopus laevis. Development, 116 (4). pp. 901-914. ISSN 0950-1991.

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In Xenopus, convergence and extension are produced by active intercalation of the deep mesodermal cells between one another along the mediolateral axis (mediolateral cell intercalation), to form a narrower, longer array. The cell motility driving this intercalation is poorly understood. A companion paper shows that the endodermal epithelium organizes the outermost mesodermal cells immediately beneath it to undergo convergence and extension, and other evidence suggests that these deep cells are the most active participants in mediolateral intercalation (Shih, J. and Keller, R. (1992) Development 116, 887–899). In this paper, we shave off the deeper layers of mesodermal cells, which allows us to observe the protrusive activity of the mesodermal cells next to the organizing epithelium with high resolution video microscopy. These mesodermal cells divide in the early gastrula and show rapid, randomly directed protrusive activity. At the early midgastrula stage, they begin to express a characteristic sequence of behaviors, called mediolateral intercalation behavior (MIB): (1) large, stable, filiform and lamelliform protrusions form in the lateral and medial directions, thus making the cells bipolar; (2) these protrusions are applied directly to adjacent cell surfaces and exert traction on them, without contact inhibition; (3) as a result, the cells elongate and align parallel to the mediolateral axis and perpendicular to the axis of extension; (4) the elongate, aligned cells intercalate between one another along the mediolateral axis, thus producing a longer, narrower array. Explants of essentially a single layer of deep mesodermal cells, made at stage 10.5, converge and extend by mediolateral intercalation. Thus by stage 10.5 (early midgastrula), expression of MIB among deep mesodermal cells is physiologically and mechanically independent of the organizing influence of the endodermal epithelium, described previously (Shih, J. and Keller, R. (1992) Development 116 887–899), and is the fundamental cell motility underlying mediolateral intercalation and convergence and extension of the body axis.

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Additional Information:© 1992 The Company of Biologists Limited. Accepted 28 August 1992. This work was supported by NSF grant DCB89052 and NIH grant HD25594. We thank J. P. Trinkaus for his inspiration, encouragement, and pioneering work in morphogenesis. We also thank Paul Wilson for his pointed criticism and support. Finally, we thank Jessica Bolker, Mary Constance Lane, Susan Purcell, Amy Sater and Barbara Stebbins for their insightful comments and suggestions.
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Subject Keywords:gastrulation, Xenopus, cell motility, protrusive activity, cell alignment, cell intercalation, morphogenesis
Issue or Number:4
Record Number:CaltechAUTHORS:20120322-130911674
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29812
Deposited By: Tony Diaz
Deposited On:17 Apr 2012 22:21
Last Modified:03 Oct 2019 03:44

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