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Convective tissue movements play a major role in avian endocardial morphogenesis

Aleksandrova, Anastasiia and Czirόk, Andras and Szabό, Andras and Filla, Michael B. and Hossain, M. Julius and Whelan, Paul F. and Lansford, Rusty and Rongish, Brenda J. (2012) Convective tissue movements play a major role in avian endocardial morphogenesis. Developmental Biology, 363 (2). pp. 348-361. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20120323-110907148

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Abstract

Endocardial cells play a critical role in cardiac development and function, forming the innermost layer of the early (tubular) heart, separated from the myocardium by extracellular matrix (ECM). However, knowledge is limited regarding the interactions of cardiac progenitors and surrounding ECM during dramatic tissue rearrangements and concomitant cellular repositioning events that underlie endocardial morphogenesis. By analyzing the movements of immunolabeled ECM components (fibronectin, fibrillin-2) and TIE1 positive endocardial progenitors in time-lapse recordings of quail embryonic development, we demonstrate that the transformation of the primary heart field within the anterior lateral plate mesoderm (LPM) into a tubular heart involves the precise co-movement of primordial endocardial cells with the surrounding ECM. Thus, the ECM of the tubular heart contains filaments that were associated with the anterior LPM at earlier developmental stages. Moreover, endocardial cells exhibit surprisingly little directed active motility, that is, sustained directed movements relative to the surrounding ECM microenvironment. These findings point to the importance of large-scale tissue movements that convect cells to the appropriate positions during cardiac organogenesis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2011.12.036DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160611014643PublisherArticle
ORCID:
AuthorORCID
Hossain, M. Julius0000-0002-3377-7831
Lansford, Rusty0000-0002-2159-3699
Additional Information:© 2012 Elsevier Inc. Received 1 July 2011. Revised 13 December 2011. Accepted 14 December 2011. Available online 4 January 2012. The authors would like to thank Dr. Charles Little for his insightful comments and for his assistance preparing the schematics. We also wish to thank Ms. Pat St John for her expert technical assistance in optimizing the conditions for cryosectioning avian embryonic hearts. This work was supported by NIH grants HL085694 (BJR); HL087136 (AC); the Hungarian Research Fund OTKA K72664 (AC); the G. Harold & Leila Y. Mathers Charitable Foundation (BJR, AC), a Faculty Scholar Award supported by P20 RR016475 from the NCRR (BJR) and a Biomedical Research Training Program award (AA).
Funders:
Funding AgencyGrant Number
NIHHL085694
NIHHL087136
Hungarian Scientific Research Fund (OTKA)K72664
G. Harold and Leila Y. Mathers Charitable FoundationUNSPECIFIED
NIHP20 RR016475
NIH Predoctoral FellowshipUNSPECIFIED
Subject Keywords:Endocardium; Embryo; Morphogenesis; Quail; Time-lapse; Cardiovascular; Imaging
Issue or Number:2
Record Number:CaltechAUTHORS:20120323-110907148
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120323-110907148
Official Citation:Anastasiia Aleksandrova, Andras Czirók, Andras Szabó, Michael B. Filla, M. Julius Hossain, Paul F. Whelan, Rusty Lansford, Brenda J. Rongish, Convective tissue movements play a major role in avian endocardial morphogenesis, Developmental Biology, Volume 363, Issue 2, 15 March 2012, Pages 348-361, ISSN 0012-1606, 10.1016/j.ydbio.2011.12.036. (http://www.sciencedirect.com/science/article/pii/S0012160611014643)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29824
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:23 Mar 2012 21:35
Last Modified:09 Mar 2020 13:18

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