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Cytokinin signaling as a positional cue for patterning the apical–basal axis of the growing Arabidopsis shoot meristem

Chickarmane, Vijay S. and Gordon, Sean P. and Tarr, Paul T. and Heisler, Marcus G. and Meyerowitz, Elliot M. (2012) Cytokinin signaling as a positional cue for patterning the apical–basal axis of the growing Arabidopsis shoot meristem. Proceedings of the National Academy of Sciences of the United States of America, 109 (10). pp. 4002-4007. ISSN 0027-8424. PMCID PMC3309735. https://resolver.caltech.edu/CaltechAUTHORS:20120327-065955396

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Abstract

The transcription factor WUSCHEL (WUS) acts from a well-defined domain within the Arabidopsis thaliana shoot apical meristem (SAM) to maintain a stem cell niche. A negative-feedback loop involving the CLAVATA (CLV) signaling pathway regulates the number of WUS-expressing cells and provides the current paradigm for the homeostatic maintenance of stem cell numbers. Despite the continual turnover of cells in the SAM during development, the WUS domain remains patterned at a fixed distance below the shoot apex. Recent work has uncovered a positive-feedback loop between WUS function and the plant hormone cytokinin. Furthermore, loss of function of the cytokinin biosynthetic gene, LONELY GUY (LOG), results in a wus-like phenotype in rice. Herein, we find the Arabidopsis LOG4 gene is expressed in the SAM epidermis. We use this to develop a computational model representing a growing SAM to suggest the plausibility that apically derived cytokinin and CLV signaling, together, act as positional cues for patterning the WUS domain within the stem cell niche. Furthermore, model simulations backed by experimental data suggest a previously unknown negative feedback between WUS function and cytokinin biosynthesis in the Arabidopsis SAM epidermis. These results suggest a plausible dynamic feedback principle by which the SAM stem cell niche is patterned.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.1200636109DOIArticle
http://www.pnas.org/content/109/10/4002PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3309735/PubMed CentralArticle
http://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1200636109/-/DCSupplementalPubMed CentralSupporting Information
ORCID:
AuthorORCID
Heisler, Marcus G.0000-0001-5644-8398
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2012 by the National Academy of Sciences. Contributed by Elliot M. Meyerowitz, January 20, 2012 (sent for review May 9, 2011). Author contributions: V.S.C., S.P.G., and P.T.T. designed research; S.P.G. and P.T.T. performed research; M.G.H. contributed new reagents/analytic tools; V.S.C., S.P.G., P.T.T., and E.M.M. analyzed data; and V.S.C., S.P.G., P.T.T., and E.M.M. wrote the paper. The authors declare no conflict of interest. V.S.C., S.P.G., and P.T.T. contributed equally to this work. We thank Adrienne Roeder, Kaoru Sugimoto, Yun Zhou, and members of the Computable Plant group (http://computableplant.org/) for comments on themanuscript; and A. Garda for technical support. This work was supported by National Science Foundation Grant IOS-0846192 (to E.M.M.) and National Institutes of Health National Research Service Award Postdoctoral Fellowship F32-GM090534 (to P.T.T.). The authors declare no conflict of interest. This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1200636109/-/DCSupplemental.
Funders:
Funding AgencyGrant Number
NSFIOS-0846192
NIH Postdoctoral FellowshipF32-GM090534
Subject Keywords:signal transduction; cell division
Issue or Number:10
PubMed Central ID:PMC3309735
Record Number:CaltechAUTHORS:20120327-065955396
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120327-065955396
Official Citation:Vijay S. Chickarmane, Sean P. Gordon, Paul T. Tarr, Marcus G. Heisler, and Elliot M. Meyerowitz Cytokinin signaling as a positional cue for patterning the apical–basal axis of the growing Arabidopsis shoot meristem PNAS 2012 109 (10) 4002-4007; published ahead of print February 15, 2012, doi:10.1073/pnas.1200636109
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29850
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:27 Mar 2012 14:24
Last Modified:03 Oct 2019 03:45

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