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Genomic Analysis Reveals Multiple [FeFe] Hydrogenases and Hydrogen Sensors Encoded by Treponemes from the H_2-Rich Termite Gut

Ballor, Nicholas R. and Paulsen, Ian and Leadbetter, Jared R. (2012) Genomic Analysis Reveals Multiple [FeFe] Hydrogenases and Hydrogen Sensors Encoded by Treponemes from the H_2-Rich Termite Gut. Microbial Ecology, 63 (2). pp. 282-294. ISSN 0095-3628. https://resolver.caltech.edu/CaltechAUTHORS:20120330-131155134

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Abstract

We have completed a bioinformatic analysis of the hydrogenases encoded in the genomes of three termite gut treponeme isolates: hydrogenotrophic, homoacetogenic Treponema primitia strains ZAS-1 and ZAS-2, and the hydrogen-producing, sugar-fermenting Treponema azotonutricium ZAS-9. H_2 is an important free intermediate in the breakdown of wood by termite gut microbial communities, reaching concentrations in some species exceeding those measured for any other biological system. The spirochetes encoded 4, 8, and 5 [FeFe] hydrogenase-like proteins, identified by their H domains, respectively, but no other recognizable hydrogenases. The [FeFe] hydrogenases represented many sequence families previously proposed in an analysis of termite gut metagenomic data. Each strain encoded both putative [FeFe] hydrogenase enzymes and evolutionarily related hydrogen sensor/transducer proteins likely involved in phosphorelay or methylation pathways, and possibly even chemotaxis. A new family of [FeFe] hydrogenases (FDH-Linked) is proposed that may form a multimeric complex with formate dehydrogenase to provide reducing equivalents for reductive acetogenesis in T. primitia. The many and diverse [FeFe] hydrogenase-like proteins encoded within the sequenced genomes of the termite gut treponemes has enabled the discovery of a putative new class of [FeFe] hydrogenase proteins potentially involved in acetogenesis and furthered present understanding of many families, including sensory, of H domain proteins beyond what was possible through the use of fragmentary termite gut metagenome sequence data alone, from which they were initially defined.


Item Type:Article
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URLURL TypeDescription
http://dx.doi.org/10.1007/s00248-011-9922-8DOIArticle
https://rdcu.be/bzW9pPublisherFree ReadCube access
ORCID:
AuthorORCID
Leadbetter, Jared R.0000-0002-7033-0844
Additional Information:© 2011 Springer Science Business Media, LLC. Received: 15 March 2011. Accepted: 18 July 2011. Published online: 3 August 2011. This research was supported by a grant from the NSF (MCB-0523267) and an NSF Graduate Student Research Fellowship (to NRB). The draft shotgun genome sequencing of T. primitia ZAS-1 was performed by Richard White and Stephen Quake at Stanford University, for which we are extremely grateful. We would like to thank our laboratory colleagues for their insightful comments during the preparation of this manuscript.
Funders:
Funding AgencyGrant Number
NSFMCB-0523267
NSF Graduate Research FellowshipUNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20120330-131155134
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120330-131155134
Official Citation:Ballor, N.R., Paulsen, I. & Leadbetter, J.R. Microb Ecol (2012) 63: 282. https://doi.org/10.1007/s00248-011-9922-8
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29913
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:30 Mar 2012 20:34
Last Modified:03 Oct 2019 03:45

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