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Flexible microelectrode arrays to interface epicardial electrical signals with intracardial calcium transients in zebrafish hearts

Yu, Fei and Zhao, Yu and Gu, Jie and Quigley, Katherine L. and Chi, Neil C. and Tai, Yu-Chong and Hsiai, Tzung K. (2012) Flexible microelectrode arrays to interface epicardial electrical signals with intracardial calcium transients in zebrafish hearts. Biomedical Microdevices, 14 (2). pp. 357-366. ISSN 1387-2176. PMCID PMC3322508. https://resolver.caltech.edu/CaltechAUTHORS:20120417-091746701

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Abstract

The zebrafish (Danio rerio) is an emerging genetic model for regenerative medicine. In humans, myocardial infarction results in the irreversible loss of cardiomyocytes. However, zebrafish hearts fully regenerate after a 20% ventricular resection, without either scarring or arrhythmias. To study this cardiac regeneration, we developed implantable flexible multi-microelectrode membrane arrays that measure the epicardial electrocardiogram signals of zebrafish in real-time. The microelectrode electrical signals allowed for a high level of both temporal and spatial resolution (~20 μm), and the signal to noise ratio of the epicardial ECG was comparable to that of surface electrode ECG (7.1 dB vs. 7.4 dB, respectively). Processing and analysis of the signals from the microelectrode array demonstrated distinct ECG signals: namely, atrial conduction (P waves), ventricular contraction (QRS), and ventricular repolarization (QT interval). The electrical signals were in synchrony with optically measured Calcium concentration gradients in terms of d[Ca^(2+)]/dt at both whole heart and tissue levels. These microelectrodes therefore provide a real-time analytical tool for monitoring conduction phenotypes of small vertebral animals with a high temporal and spatial resolution.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1007/s10544-011-9612-9DOIArticle
https://rdcu.be/bXA6uPublisherFree ReadCube access
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322508PubMed CentralArticle
ORCID:
AuthorORCID
Tai, Yu-Chong0000-0001-8529-106X
Hsiai, Tzung K.0000-0003-1734-0792
Additional Information:© 2011 Springer Science+Business Media, LLC. Published online: 29 November 2011. The authors would like to express gratitude for Dr. Fuhua Chen from UCLA School of Medicine for providing the Calcium voltage mapping. This project was supported by the American Heart Association Pre-Doctoral Fellowship (11PRE7370088) (FY), National Institutes of Health, National Heart Lung and Blood Institute (HL083015) (TKH), HL091302 (TKH), (HL104239) (NCC) and National Institute of Child Health & Human Development (HD069305) (NCC and TKH).
Funders:
Funding AgencyGrant Number
NIHHL083015
American Heart Association11PRE7370088
NIHHL091302
NIHHL104239
NIHHD069305
National Heart Lung and Blood InstituteUNSPECIFIED
National Institute of Child Health and Human Development (NICHD)UNSPECIFIED
Subject Keywords:Zebrafish hearts; Flexible electronics; ECG; Cardiac conduction; Calcium waves
Issue or Number:2
PubMed Central ID:PMC3322508
Record Number:CaltechAUTHORS:20120417-091746701
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120417-091746701
Official Citation:Yu, F., Zhao, Y., Gu, J. et al. Biomed Microdevices (2012) 14: 357. https://doi.org/10.1007/s10544-011-9612-9
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:30122
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:17 Apr 2012 18:03
Last Modified:25 Nov 2019 23:39

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