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Avian neural crest cell attachment to laminin: involvement of divalent cation dependent and independent integrins

Lallier, Thomas and Bronner-Fraser, Marianne (1991) Avian neural crest cell attachment to laminin: involvement of divalent cation dependent and independent integrins. Development, 113 (4). pp. 1069-1084. ISSN 0950-1991.

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The mechanisms of neural crest cell interaction with laminin were explored using a quantitative cell attachment assay. With increasing substratum concentrations, an increasing percentage of neural crest cells adhere to laminin. Cell adhesion at all substratum concentrations was inhibited by the CSAT antibody, which recognizes the chick β_1 subunit of integrin, suggesting that β_(1-)integrins mediate neural crest cell interactions with laminin. The HNK-1 antibody, which recognizes a carbohydrate epitope, inhibited neural crest cell attachment to laminin at low coating concentrations (>1 µg ml^(-1); Low-LM), but not at high coating concentration of laminin (10 µg ml^(-1); High-LM). Attachment to Low-LM occurred in the absence of divalent cations, whereas attachment to High-LM required >0.1 mM Ca^(2+) or Mn^(2+). Neural crest cell adherence to the E8 fragment of laminin, derived from its long arm, was similar to that on intact laminin at high and low coating concentrations, suggesting that this fragment contains the neural crest cell binding site(s). The HNK-1 antibody recognizes a protein of 165,000 Mr which is also found in immunoprecipitates using antibodies against the β_1 subunit of integrin and is likely to be an integrin alpha subunit or an integrin-associated protein. Our results suggest that the HNK-1 epitope on neural crest cells is present on or associated with a novel or differentially glycosylated form of β_(1-)integrin, which recognizes laminin in the apparent absence of divalent cations. We conclude that neural crest cells have at least two functionally independent means of attachment to laminin which are revealed at different substratum concentrations and/or conformations of laminin.

Item Type:Article
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Lallier, Thomas0000-0001-6577-6676
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1991 Company of Biologists Limited. Accepted 28 August 1991. We thank Drs Scott Fraser and Roberto Perris for helpful comments on the manuscript and invaluable discussion. We also would like to thank Dr Mats Paulsson for his generous gift of laminin fragments and the laminin-nidogen complex. This study was supported by USPHS Grant HD-15527.
Funding AgencyGrant Number
Subject Keywords:extracellular matrix receptors, cell adhesion, laminin, integrin, neural crest, quail
Issue or Number:4
Record Number:CaltechAUTHORS:20120419-081816394
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Official Citation:T. Lallier and M. Bronner-Fraser Avian neural crest cell attachment to laminin: involvement of divalent cation dependent and independent integrins Development 1991 113:1069-1084
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:30194
Deposited By: Ruth Sustaita
Deposited On:19 Apr 2012 17:22
Last Modified:23 Dec 2022 17:04

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