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Chemoenzymatic Probes for Detecting and Imaging Fucose-α(1-2)-galactose Glycan Biomarkers

Chaubard, Jean-Luc and Krishnamurthy, Chithra and Yi, Wen and Smith, David F. and Hsieh-Wilson, Linda C. (2012) Chemoenzymatic Probes for Detecting and Imaging Fucose-α(1-2)-galactose Glycan Biomarkers. Journal of the American Chemical Society, 134 (10). pp. 4489-4492. ISSN 0002-7863. PMCID PMC3303202. https://resolver.caltech.edu/CaltechAUTHORS:20120420-103240242

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Abstract

The disaccharide motif fucose-α(1-2)-galactose (Fucα(1-2)Gal) is involved in many important physiological processes, such as learning and memory, inflammation, asthma, and tumorigenesis. However, the size and structural complexity of Fucα(1-2)Gal-containing glycans have posed a significant challenge to their detection. We report a new chemoenzymatic strategy for the rapid, sensitive detection of Fucα(1-2)Gal glycans. We demonstrate that the approach is highly selective for the Fucα(1-2)Gal motif, detects a variety of complex glycans and glycoproteins, and can be used to profile the relative abundance of the motif on live cells, discriminating malignant from normal cells. This approach represents a new potential strategy for biomarker detection and expands the technologies available for understanding the roles of this important class of carbohydrates in physiology and disease.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja211312uDOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja211312uPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303202/PubMed CentralArticle
ORCID:
AuthorORCID
Hsieh-Wilson, Linda C.0000-0001-5661-1714
Additional Information:© 2012 American Chemical Society. ACS AuthorChoice. Received: December 2, 2011. Publication Date (Web): February 16, 2012. We thank Rochelle Diamond at the Caltech Flow Cytometry Cell Sorting Facility, Dr. Mona Shahgholi in the CCE Division Mass Spectrometry Facility, and The Consortium for Functional Glycomics, Core D and H (NIGMS GM62116), and also Dr. Peng George Wang for providing the BgtA vector. This work was supported by grants from the NIH (R01 GM084724 to L.C.H.−W. and NIH/NRSA 5T32 GM07616 to C.K. and J.C.).
Funders:
Funding AgencyGrant Number
NIHGM62116
NIHR01 GM084724
NIH Predoctoral Fellowship5-T32-GM07616
Issue or Number:10
PubMed Central ID:PMC3303202
Record Number:CaltechAUTHORS:20120420-103240242
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120420-103240242
Official Citation:Chemoenzymatic Probes for Detecting and Imaging Fucose-α(1-2)-galactose Glycan Biomarkers Jean-Luc Chaubard, Chithra Krishnamurthy, Wen Yi, David F. Smith, and Linda C. Hsieh-Wilson Journal of the American Chemical Society 2012 134 (10), 4489-4492
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:30218
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:20 Apr 2012 18:10
Last Modified:03 Oct 2019 03:48

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