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Developmental regulation of expression of the lactate dehydrogenase (LDH) multigene family during mouse spermatogenesis

Thomas, Kelwyn and Del Mazo, Jesus and Eversole, Pam and Bellvé, Anthony and Hiraoka, Yukihiro and Li, Steven S. L. and Simon, Melvin (1990) Developmental regulation of expression of the lactate dehydrogenase (LDH) multigene family during mouse spermatogenesis. Development, 109 (2). pp. 483-493. ISSN 0950-1991.

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Expression of the Lactate Dehydrogenase (LDH) genes during various stages of spermatogenesis was studied by using a combination of Northern blot analyses and in situ hybridization techniques. These studies have indicated that developmentally programmed expression of all three functional LDH genes occurs during differentiation of germ cells. The LDH/C (ldh-3) gene was expressed exclusively during meiosis and spermiogenesis, beginning in leptotene/zygotene spermatocytes and continuing through to the elongated spermatids. LDH/C (ldh-3) gene expression was accompanied by transient expression of the LDH/A (ldh-1) gene in pachytene spermatocytes and round spermatids. The LDH/B (ldh-2) gene was expressed mainly in Sertoli and spermatogonial cells. By using somatic cell hybrids, the LDH/C (ldh-3) gene has been mapped to mouse chromosome 7, establishing that it is syntenic with the LDH/A (ldh-1) gene locus. Experimental observations made in this study provide new insight into the order and sequence of events involved in the regulation of gene expression of the LDH gene family during spermatogenesis.

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Additional Information:© 1990 Company of Biologists Limited. Accepted 14 March 1990. We thank Drs Barbara Wold and James Bonner for critical reading of the manuscript, Drs Akai, Hiraoka and Saiki for the LDH/A, LDH/B and LDH/C cDNAs, and Dr Tomashifsky for mouse germ cell purifications. Discussions and advice from several coworkers in the Simon lab were greatly appreciated. Thanks to Joyce Ito and Cathy Blagg for assistance with typing. This research was funded by an American Cancer Society grant to M.I.S., National Institute of Child Health and Human Development Grants R.01 HD 05077 and HD 21825 to A.R.B. J.M. is a Visiting Associate supported by a M.E.C./Fulbright fellowship.
Funding AgencyGrant Number
American Cancer SocietyUNSPECIFIED
National Institute of Child Health and Human DevelopmentR.01 HD 05077
National Institute of Child Health and Human DevelopmentHD 21825
MEC/Fulbright fellowshipUNSPECIFIED
Subject Keywords:spermatogenesis; LDH gene expression; meiosis
Issue or Number:2
Record Number:CaltechAUTHORS:20120425-134307051
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:30358
Deposited By: Jason Perez
Deposited On:25 Apr 2012 22:15
Last Modified:03 Oct 2019 03:49

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