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Structure of the inverted terminal repetition of adenovirus type 2 DNA

Wu, Madeline and Roberts, Richard J. and Davidson, Norman (1977) Structure of the inverted terminal repetition of adenovirus type 2 DNA. Journal of Virology, 21 (2). pp. 766-777. ISSN 0022-538X.

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Several secondary structure features involving the ends of single strands of adenovirus type 2 DNA have been studied by electron microscopy by both the gene 32-ethidium bromide technique and a modification of the standard formamide-cytochrome c technique. A duplex stem of length 115 +/- 10 nucleotide pairs due to pairing between the two members of the inverted terminal repetition is observed in the single-stranded circles that form upon annealing single-stranded linear molecules. This duplex stem is shown to lie at the ends of the DNA by using several reference markers: (i) a newly discovered secondary structure feature (a loop of length ca. 500 nucleotides with a 20-nucleotide pair duplex stem) that maps 73% of the full length from the left end of the molecule and (ii) a duplex region due to a hybridized restriction fragment. There is also some secondary structure within each end of linear single strands. There is some variation in the morphology of the end strucures, and we propose that these involve base pairing, as in a tRNA clover leaf, rather than an exact single hairpin-type inverted repeat. These observations are consistent with the hypothesis that there is a foldback structure at the 3' ends of the DNA that functions as a primer for the initiation of replication.

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Additional Information:Copyright © 1977 by the American Society for Microbiology. Received for publication 5 August 1976 We are grateful to J. D. Watson for stimulating advice and comments. This research was supported by Public Health Service grants GM-20927 and GM-10991 from the National Institute of General Medical Sciences to N.D. and R.R., respectively.
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ID Code:3049
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Deposited On:12 May 2006
Last Modified:02 Oct 2019 22:59

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