Sigmund, Orit and Naort, Zvi and Anderson, David J. and Stein, Reuven (1990) Effect of nerve growth factor and fibroblast growth factor on SCG10 and c-fos expression and neurite outgrowth in protein kinase C-depleted PC12 cells. Journal of Biological Chemistry, 265 (4). pp. 2257-2261. ISSN 0021-9258. https://resolver.caltech.edu/CaltechAUTHORS:20120509-132826402
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Abstract
The role of protein kinase C (PKC) in mediating nerve growth factor (NGF) or basic fibroblast growth factor (bFGF)-stimulated SCG10 and c-fos expression as well as neurite outgrowth was studied in PC12 cells. Activators of PKC such as phorbol 12-myristate 13-acetate (PMA) or 1-oleoyl 2-acetyl glycerol mimicked the stimulatory effect of NGF and bFGF on SCG10 mRNA levels. Induction involved a protein synthesis-dependent mechanism and was maximal within 12-24 h of exposure. Chronic treatment of the cells with PMA for up to 8 days resulted in a substantial decrease (approximately 90%) in total PKC activity in the continued presence of PMA. PKC depletion did not affect NGF- or bFGF-stimulated SCG10 mRNA induction and bFGF-stimulated c-fos mRNA induction. However, NGF-stimulated c-fos mRNA induction was attenuated. In addition, induction of neurite outgrowth was not abolished in PKC-depleted cells. The results imply that PKC is not involved in NGF- and bFGF-stimulated SCG10 mRNA induction and neurite outgrowth. Furthermore, while the effect of bFGF on c-fos mRNA induction is PKC-independent, that of NGF is mediated by PKC-dependent and -independent pathways.
| Item Type: | Article | ||||||
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| Additional Information: | © 1990 American Society for Biochemistry and Molecular Biology, Inc. Received for publication, June 5, 1989. This work was supported by United States-Israel Binational Science Foundation Grant 86-00246/1. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. | ||||||
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| Issue or Number: | 4 | ||||||
| Record Number: | CaltechAUTHORS:20120509-132826402 | ||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20120509-132826402 | ||||||
| Official Citation: | Effect of nerve growth factor and fibroblast growth factor on SCG10 and c-fos expression and neurite outgrowth in protein kinase C-depleted PC12 cells. O Sigmund, Z Naor, D J Anderson, and R Stein J. Biol. Chem. 1990 265: 2257-2261. | ||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
| ID Code: | 31382 | ||||||
| Collection: | CaltechAUTHORS | ||||||
| Deposited By: | Ruth Sustaita | ||||||
| Deposited On: | 09 May 2012 22:34 | ||||||
| Last Modified: | 03 Oct 2019 03:51 |
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