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Lipopolysaccharide Is a Potent Monocyte/Macrophage-specific Stimulator of Human Immunodeficiency Virus Type 1 Expression

Pomerantz, Roger J. and Feinberg, Mark B. and Trono, Didier and Baltimore, David (1990) Lipopolysaccharide Is a Potent Monocyte/Macrophage-specific Stimulator of Human Immunodeficiency Virus Type 1 Expression. Journal of Experimental Medicine, 172 (1). pp. 253-261. ISSN 0022-1007. PMCID PMC2188186. doi:10.1084/jem.172.1.253.

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Lipopolysaccharide (LPS) potently stimulates human immunodeficiency virus type 1-long terminal repeat (HIV-1-LTR) CAT constructs transfected into monocyte/macrophage-like cell lines but not a T cell line. This effect appears to be mediated through the induction of nuclear factor kappa B (NF-kappa B). Electrophoretic mobility shift assays demonstrate that LPS induces a DNA binding activity indistinguishable from NF-kappa B in U937 and THP-1 cells. LPS is also shown to dramatically increase HIV-1 production from a chronically infected monocyte/macrophage-like cloned cell line, U1, which produces very low levels of HIV-1 at baseline. The stimulation of viral production from this cell line occurs only if these cells are treated with granulocyte/macrophage colony-stimulating factor (GM-CSF) before treatment with LPS. This stimulation of HIV-1 production is correlated with an increase in the level of HIV-1 RNA and and activation of NF-kappa B. LPS is not able to induce HIV-1 production in a cloned T cell line. The effect of LPS on HIV-1 replication occurs at picogram per milliliter concentrations and may be clinically significant in understanding the variability of the natural history of HIV-1 infection.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1990 Rockefeller University Press. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at, or updates thereof. Received for publication 23 February 1990. Published July 1, 1990. We thank Drs . Sankar Ghosh, Mark Muesing, Anthony De Franco, and Towia Libermann for critical discussions. We thank Dr. Michael Lenardo for providing some oligonucleotides and Dr. Jacqueline Pierce for providing some of the plasmids used in these studies. We also thank Patricia Waddy for excellent secretarial assistance. R. J. Pomerantz is funded under Physician Scientist Award AI-00930. This work was supported in part by U.S. Public Health Service grants AI-26463 and HIA3510.
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PubMed Central ID:PMC2188186
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Official Citation:Lipopolysaccharide is a potent monocyte/macrophage-specific stimulator of human immunodeficiency virus type 1 expression. R J Pomerantz, M B Feinberg, D Trono, and D Baltimore 172:253-261. doi:10.1084/jem.172.1.253
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:31387
Deposited By: Ruth Sustaita
Deposited On:09 May 2012 21:31
Last Modified:09 Nov 2021 19:52

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