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Topologically Restricted Appearance in the Developing Chick Retinotectal System of Bravo, a Neural Surface Protein: Experimental Modulation by Environmental Cues

de la Rosa, Enrique J. and Kayyem, Jon F. and Roman, Janet M. and Stierhof, York-Dieter and Dreyer, William J. and Schwarz, Uli (1990) Topologically Restricted Appearance in the Developing Chick Retinotectal System of Bravo, a Neural Surface Protein: Experimental Modulation by Environmental Cues. Journal of Cell Biology, 111 (6). pp. 3087-3096. ISSN 0021-9525. PMCID PMC2116392. doi:10.1083/jcb.111.6.3087.

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A novel neural surface protein, Bravo, shows a pattern of topological restriction in the embryonic chick retinotectal system. Bravo is present on the developing optic fibers in the retina; however, retinal axons in the tectum do not display Bravo. The appearance of Bravo in vitro is modulated by environmental cues. Axons growing out from retinal explants on retinal basal lamina, their natural substrate, express Bravo, whereas such axons growing on collagen do not. Retinal explants provide a valuable system to characterize the mechanism of Bravo restriction, as well as the cellular signals controlling it. Bravo was identified with monoclonal antibodies from a collection generated against exposed molecules isolated by using a selective cell surface biotinylation procedure. The NH2-terminal sequence of Bravo shows similarity with L1, a neural surface molecule which is a member of the immunoglobulin superfamily. This possible relationship to L1, together with its restricted appearance, suggests an involvement of Bravo in axonal growth and guidance.

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Additional Information:© 1990 Rockefeller University Press. Received for publication 27 November 1989 and in revised form 6 September 1990. We wish to thank Naomi Barker, Jürgen Berger, Regine Braun, Silvia Deiss, Devra Ellert, Roswitha Groemke-Lutz, Victoria Kasmer, Jennifer Kwang Hee Kim, Inge Zimmermann, and the staff of the Caltech Hybridoma Facility for excellent technical assistance in different moments and aspects of the project; Ysander yon Boxberg, Dieter Diitting, and Stephan Kröger for advice about different methods; Fritz Rathjen for generous gift of many antibodies; Puola Bovolenta, Sandy Cooper, Elisabeth Pollerberg, and Margarita del Val for critical reading of the manuscript, and Ingrid Baxivanelis and Christiane Seeland-Woehrle for secretarial work. The Caltech participants also wish to thank the many members of the Max- Planck-Institut for the stimulating and pleasant discussions and hospitality during the year that the three of us spent in Tübingen, Germany. Enrique J. de la Rosa was the recipient of postdoctoral fellowships of the Alexander von Humboldt-Stiftung (Germany) and Ministerio de Educaciόn y Ciencia (Spain). Jon F. Kayyem was supported by a graduate fellowship from the Office of Naval Research (USA). This work was funded by Institutional funding by Max-Planck- Gesellschafi, Lucille P. Markey Charitable Trust Grant in Developmental Biology to Caltech, and The National Institutes of Health (EY 07752).
Funding AgencyGrant Number
Alexander von Humboldt StiftungUNSPECIFIED
Ministerio de Educaciόn y Ciencia (MEC)UNSPECIFIED
Office of Naval Research (ONR)UNSPECIFIED
Lucille P. Markey Charitable TrustUNSPECIFIED
NIHEY 07752
Issue or Number:6
PubMed Central ID:PMC2116392
Record Number:CaltechAUTHORS:20120509-152854854
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Official Citation:Topologically restricted appearance in the developing chick retinotectal system of Bravo, a neural surface protein: experimental modulation by environmental cues. E J de la Rosa, J F Kayyem, J M Roman, Y D Stierhof, W J Dreyer, and U Schwarz 111:3087-3096. doi:10.1083/jcb.111.6.3087
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:31391
Deposited By: Ruth Sustaita
Deposited On:10 May 2012 18:28
Last Modified:09 Nov 2021 19:52

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