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A vital dye analysis of the timing and pathways of avian trunk neural crest cell migration

Serbedzija, George N. and Fraser, Scott E. and Bronner-Fraser, Marianne E. (1989) A vital dye analysis of the timing and pathways of avian trunk neural crest cell migration. Development, 106 (4). pp. 809-816. ISSN 0950-1991.

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To permit a more detailed analysis of neural crest cell migratory pathways in the chick embryo, neural crest cells were labelled with a nondeleterious membrane intercalating vital dye, DiI. All neural tube cells with endfeet in contact with the lumen, including premigratory neural crest cells, were labelled by pressure injecting a solution of DiI into the lumen of the neural tube. When assayed one to three days later, migrating neural crest cells, motor axons, and ventral root cells were the only cells types external to the neural tube labelled with DiI. During the neural crest cell migratory phase, distinctly labelled cells were found along: (1) a dorsolateral pathway, under the epidermis, as well adjacent to and intercalating through the dermamyotome; and (2) a ventral pathway, through the rostral portion of each sclerotome and around the dorsal aorta as described previously. In contrast to those cells migrating through the sclerotome, labelled cells on the dorsolateral pathway were not segmentally arranged along the rostrocaudal axis. DiI-labelled cells were observed in all truncal neural crest derivatives, including subepidermal presumptive pigment cells, dorsal root ganglia, and sympathetic ganglia. By varying the stage at which the injection was performed, neural crest cell emigration at the level of the wing bud was shown to occur from stage 13 through stage 22. In addition, neural crest cells were found to populate their derivatives in a ventral-to-dorsal order, with the latest emigrating cells migrating exclusively along the dorsolateral pathway.

Item Type:Article
Related URLs:
URLURL TypeDescription
Fraser, Scott E.0000-0002-5377-0223
Bronner-Fraser, Marianne E.0000-0003-4274-1862
Additional Information:© 1989 Company of Biologists Ltd. Accepted 9 May 1989. We thank Dr Andrew Lumsden for critical reading of the manuscript and Scott Burgan for excellent technical assistance. This work was supported by BNS 8608356 (SEF), HD 25138-01, a grant from the Muscular Dystrophy Association (MDA), a McKnight Scholar Award, and a gift from the Monsanto Corporation. MBF is a Sloan Foundation Fellow.
Funding AgencyGrant Number
NSFBNS 8608356
NIHHD 25138-01
Muscular Dystrophy AssociationUNSPECIFIED
McKnight FoundationUNSPECIFIED
Monsanto CorporationUNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
Subject Keywords:DiI; microinjection; pigment cells; cell migration; neural crest; vital dye
Issue or Number:4
Record Number:CaltechAUTHORS:20120510-141811381
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:31420
Deposited On:10 May 2012 23:22
Last Modified:03 Oct 2019 03:51

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