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Signatures of unfolding in the early stages of protein denaturation

Gray, Harry B. and Winkler, Jay R. and Kozak, John J. (2012) Signatures of unfolding in the early stages of protein denaturation. Molecular Physics, 110 (7). pp. 419-429. ISSN 0026-8976. doi:10.1080/00268976.2011.651168.

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A comparative study of the early stages of unfolding of five proteins: cyt c, c-b_(562), cyt c′, azurin, and lysozyme is reported. From crystallographic data, helical regions and intervening non-helical (or ‘turning’) regions are identified in each. Exploiting a previously introduced geometrical model, the paper describes quantitatively the stepwise extension of a polypeptide chain subject to the geometrical constraint that the spatial relationship among the residues of each triplet is fixed by native-state crystallographic data. Despite differences among the above-cited proteins, remarkable universality of behavior is found in the early stages of unfolding. At the very earliest stages, internal residues in each helical region have a common unfolding history; the terminal residues, however, are extraordinarily sensitive to structural perturbations. Residues in non-helical sections of the polypeptide unfold after residues in the internal helical regions, but with increasing steric perturbation playing a dominant role in advancing denaturation.

Item Type:Article
Related URLs:
URLURL TypeDescription
Gray, Harry B.0000-0002-7937-7876
Winkler, Jay R.0000-0002-4453-9716
Additional Information:© 2012 Taylor & Francis. Received 30 September 2011; final version received 14 December 2011. Available online: 11 Apr 2012.
Subject Keywords: protein unfolding, denaturation, cytochrome c , cytochrome c′, cytochrome c-b562 , azurin, lysozyme
Issue or Number:7
Record Number:CaltechAUTHORS:20120514-102559323
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Official Citation: Signatures of unfolding in the early stages of protein denaturation Harry B. Gray, Jay R. Winkler, John J. Kozak Molecular Physics Vol. 110, Iss. 7, 2012
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:31447
Deposited By: Ruth Sustaita
Deposited On:15 May 2012 14:54
Last Modified:09 Nov 2021 19:53

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