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Genome-wide profiling of uncapped mRNA

Jiao, Yuling and Riechmann, José Luis (2012) Genome-wide profiling of uncapped mRNA. In: Plant Signalling Networks: Methods and Protocols. Methods in Molecular Biology. No.876. Humana Press , New York, pp. 207-216. ISBN 9781617798085.

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Gene transcripts are under extensive posttranscriptional regulation, including the regulation of their stability. A major route for mRNA degradation produces uncapped mRNAs, which can be generated by decapping enzymes, endonucleases, and small RNAs. Profiling uncapped mRNA molecules is important for the understanding of the transcriptome, whose composition is determined by a balance between mRNA synthesis and degradation. In this chapter, we describe a method to profile these uncapped mRNAs at the genome scale.

Item Type:Book Section
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URLURL TypeDescription
Jiao, Yuling0000-0002-1189-1676
Additional Information:© 2012 Springer Science+Business Media, LLC. The authors would like to thank Dr. E.M. Meyerowitz for his encouragement and advice. This work was supported by the US National Science Foundation 2010 Project Grant 0520193, by the National Natural Science Foundation of China Grant 31171159, and by the Millard and Muriel Jacobs Genetics and Genomics Laboratory at the California Institute of Technology.
Funding AgencyGrant Number
National Natural Science Foundation of China31171159
Caltech Millard and Muriel Jacobs Genetics and Genomics LaboratoryUNSPECIFIED
Subject Keywords:mRNA, Uncapping, RNA degradation, Transcriptome
Series Name:Methods in Molecular Biology
Issue or Number:876
Record Number:CaltechAUTHORS:20120521-111710017
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:31571
Deposited By: Tony Diaz
Deposited On:21 May 2012 21:55
Last Modified:09 Nov 2021 19:55

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