Jiao, Yuling and Riechmann, José Luis (2012) Genome-wide profiling of uncapped mRNA. In: Plant Signalling Networks: Methods and Protocols. Methods in Molecular Biology. No.876. Humana Press , New York, pp. 207-216. ISBN 9781617798085. https://resolver.caltech.edu/CaltechAUTHORS:20120521-111710017
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Abstract
Gene transcripts are under extensive posttranscriptional regulation, including the regulation of their stability. A major route for mRNA degradation produces uncapped mRNAs, which can be generated by decapping enzymes, endonucleases, and small RNAs. Profiling uncapped mRNA molecules is important for the understanding of the transcriptome, whose composition is determined by a balance between mRNA synthesis and degradation. In this chapter, we describe a method to profile these uncapped mRNAs at the genome scale.
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| Additional Information: | © 2012 Springer Science+Business Media, LLC. The authors would like to thank Dr. E.M. Meyerowitz for his encouragement and advice. This work was supported by the US National Science Foundation 2010 Project Grant 0520193, by the National Natural Science Foundation of China Grant 31171159, and by the Millard and Muriel Jacobs Genetics and Genomics Laboratory at the California Institute of Technology. | |||||||||
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| Subject Keywords: | mRNA, Uncapping, RNA degradation, Transcriptome | |||||||||
| Series Name: | Methods in Molecular Biology | |||||||||
| Issue or Number: | 876 | |||||||||
| DOI: | 10.1007/978-1-61779-809-2_17 | |||||||||
| Record Number: | CaltechAUTHORS:20120521-111710017 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20120521-111710017 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 31571 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Tony Diaz | |||||||||
| Deposited On: | 21 May 2012 21:55 | |||||||||
| Last Modified: | 09 Nov 2021 19:55 |
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