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DNA Electrochemistry with Tethered Methylene Blue

Pheeney, Catrina G. and Barton, Jacqueline K. (2012) DNA Electrochemistry with Tethered Methylene Blue. Langmuir, 28 (17). pp. 7063-7070. ISSN 0743-7463. PMCID PMC3398613. doi:10.1021/la300566x. https://resolver.caltech.edu/CaltechAUTHORS:20120521-152748382

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Abstract

Methylene blue (MB′), covalently attached to DNA through a flexible C_12 alkyl linker, provides a sensitive redox reporter in DNA electrochemistry measurements. Tethered, intercalated MB′ is reduced through DNA-mediated charge transport; the incorporation of a single base mismatch at position 3, 10, or 14 of a 17-mer causes an attenuation of the signal to 62 ± 3% of the well-matched DNA, irrespective of position in the duplex. The redox signal intensity for MB′–DNA is found to be least 3-fold larger than that of Nile blue (NB)–DNA, indicating that MB′ is even more strongly coupled to the π-stack. The signal attenuation due to an intervening mismatch does, however, depend on DNA film density and the backfilling agent used to passivate the surface. These results highlight two mechanisms for reduction of MB′ on the DNA-modified electrode: reduction mediated by the DNA base pair stack and direct surface reduction of MB′ at the electrode. These two mechanisms are distinguished by their rates of electron transfer that differ by 20-fold. The extent of direct reduction at the surface can be controlled by assembly and buffer conditions.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/la300566xDOIArticle
http://pubs.acs.org/doi/abs/10.1021/la300566xPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398613/PubMed CentralArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2012 American Chemical Society. Published In Issue May 01, 2012; Article ASAP April 18, 2012; Received: February 07, 2012; Revised: April 03, 2012. This research was supported by the National Institute of Health (GM61077). The authors thank N. Muren for discussions and her contributions in fabricating the multiplexed chips. This work was completed in part in the Caltech Micro Nano Fabrication Laboratory.
Funders:
Funding AgencyGrant Number
NIHGM61077
Issue or Number:17
PubMed Central ID:PMC3398613
DOI:10.1021/la300566x
Record Number:CaltechAUTHORS:20120521-152748382
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120521-152748382
Official Citation:DNA Electrochemistry with Tethered Methylene Blue Catrina G. Pheeney and Jacqueline K. Barton Langmuir 2012 28 (17), 7063-7070
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:31577
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:21 May 2012 22:55
Last Modified:09 Nov 2021 19:55

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