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Analysis of Extensive [FeFe] Hydrogenase Gene Diversity Within the Gut Microbiota of Insects Representing Five Families of Dictyoptera

Ballor, Nicholas R. and Leadbetter, Jared R. (2012) Analysis of Extensive [FeFe] Hydrogenase Gene Diversity Within the Gut Microbiota of Insects Representing Five Families of Dictyoptera. Microbial Ecology, 63 (3). pp. 586-595. ISSN 0095-3628. https://resolver.caltech.edu/CaltechAUTHORS:20120611-090321494

[img] Postscript (Figure S1 Maximum likelihood tree of all cloned family 3 [FeFe] hydrogenase sequences) - Supplemental Material
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[img] Postscript (Figure S2 Phylogram of family 3 [FeFe] hydrogenases cloned from the guts of an adult and nymph C. punctulatus samples) - Supplemental Material
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Abstract

We have designed and utilized degenerate primers in the phylogenetic analysis of [FeFe] hydrogenase gene diversity in the gut ecosystems of roaches and lower termites. H2 is an important free intermediate in the breakdown of wood by termite gut microbial communities, reaching concentrations in some species exceeding those measured for any other biological system. The primers designed target with specificity the largest group of enzymatic H domain proteins previously identified in a termite gut metagenome. “Family 3” hydrogenase sequences were amplified from the guts of lower termites, Incisitermes minor, Zootermopsis nevadensis, and Reticulitermes hesperus, and two roaches, Cryptocercus punctulatus and Periplaneta americana. Subsequent analyses revealed that all termite and Cryptocercus sequences were phylogenetically distinct from non-termiteassociated hydrogenases available from public databases. The abundance of unique sequence operational taxonomic units (as many as 21 from each species) underscores the previously demonstrated physiological importance of H2 to the gut ecosystems of these wood-feeding insects. The diversity of sequences observed might be reflective of multiple niches that the enzymes have been evolved to accommodate. Sequences cloned from Cryptocercus and the lower termite samples, all of which are wood feeding insects, clustered closely with one another in phylogenetic analyses to the exclusion of alleles from P. americana, an omnivorous cockroach, also cloned during this study. We present primers targeting a family of termite gut [FeFe] hydrogenases and provide results that are consistent with a pivotal role for hydrogen in the termite gut ecosystem and point toward unique evolutionary adaptations to the gut ecosystem.


Item Type:Article
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URLURL TypeDescription
http://dx.doi.org/10.1007/s00248-011-9941-5DOIArticle
https://rdcu.be/bzW8FPublisherFree ReadCube access
ORCID:
AuthorORCID
Leadbetter, Jared R.0000-0002-7033-0844
Additional Information:© 2012 Springer, Part of Springer Science+Business Media. Received: 15 March 2011; Accepted: 4 September 2011; Published online: 21 September 2011. This research was supported by grants from the NSF (MCB-0523267) and the DOE (DE-FG02-07ER64484) and by an NSF Graduate Student Research Fellowship (to NRB). We would like to thank our laboratory colleagues and several anonymous reviewers for their insightful comments during the preparation of this manuscript.
Funders:
Funding AgencyGrant Number
NSFMCB-0523267
Department of Energy (DOE)DE-FG02-07ER64484
NSF Graduate Research FellowshipUNSPECIFIED
Issue or Number:3
Record Number:CaltechAUTHORS:20120611-090321494
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120611-090321494
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:31869
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:13 Jun 2012 20:44
Last Modified:03 Oct 2019 03:55

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