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On-chip continuous monitoring of motile microorganisms on an ePetri platform

Lee, Seung Ah and Zheng, Guoan and Mukherjee, Nandini and Yang, Changhuei (2012) On-chip continuous monitoring of motile microorganisms on an ePetri platform. Lab on a Chip, 12 (13). pp. 2385-2390. ISSN 1473-0197. PMCID PMC3371133. https://resolver.caltech.edu/CaltechAUTHORS:20120621-135552222

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Abstract

Self-imaging Petri dish platforms with microscopy resolution, which we term ‘ePetri’, can significantly streamline cell cultures and/or other longitudinal biological studies. In this paper, we demonstrate high-resolution imaging and long-term culture of motile microorganisms in a specialized ePetri platform by taking advantage of the inherent motion. By applying a super-resolution algorithm to a set of low-resolution images of the microorganisms as they move across the sensing area of a complementary metal oxide semiconductor (CMOS) image sensor chip, we can render an improved-resolution image of the microorganisms. We perform a longitudinal study of Euglena gracilis cultured in an ePetri platform, and image-based analysis on the motion and morphology of the cells. As a miniaturized and automated culture monitoring platform, this ePetri technology can greatly improve studies and experiments with motile microorganisms.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1039/C2LC40090A DOIArticle
http://pubs.rsc.org/en/Content/ArticleLanding/2012/LC/c2lc40090aPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3371133/PubMed CentralArticle
ORCID:
AuthorORCID
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2012 The Royal Society of Chemistry. Received 24 Jan 2012, Accepted 13 Mar 2012. First published on the web 20 Mar 2012. We acknowledge funding support from the National Institute of Health (NIH) Agency Award R01AI096226-01.
Funders:
Funding AgencyGrant Number
NIHR01AI096226-01
Issue or Number:13
PubMed Central ID:PMC3371133
Record Number:CaltechAUTHORS:20120621-135552222
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120621-135552222
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32016
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Jun 2012 20:24
Last Modified:09 Mar 2020 13:19

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