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Patterns of mRNA prevalence and expression of B1 and B2 transcripts in early mouse embryos

Taylor, Kent D. and Piko, Lajos (1987) Patterns of mRNA prevalence and expression of B1 and B2 transcripts in early mouse embryos. Development, 101 (4). pp. 877-892. ISSN 0950-1991.

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Considerable evidence indicates that the 2-cell stage is a critical period of mouse embryo development when a transition from maternal to zygotic genomic control takes place. The overall changes in the structure of the mRNA population as a result of this transition were explored using a random cDNA library of 69 clones derived from late 2-cell embryos. The prevalence of the cloned sequences was analysed by dot hybridization of the cDNA clones with labelled cDNA probes synthesized to poly(A)^+ RNA from different stages of development from 1-cell through blastocyst. The number of copies of individual transcripts was quantitatively estimated by comparison to standard clones of known prevalence. About one half of the transcripts that gave a measurable reaction at the 2-cell and later stages were not represented detectably in egg RNA, suggesting that a large set of zygote-specific genes not included in the maternal gene set becomes transcriptionally active in the 2-cell embryo. Six of the cDNA clones represented B1 and B2 repeat sequences. As measured by hybridization with labelled cDNA, B1 and B2 transcripts were abundantly expressed throughout cleavage, being represented by about 10^5 to 10^6 copies per embryo. However, the developmental pattern of prevalence was different for the two transcripts suggesting that their expression is regulated independently. The results of this study corroborate previous evidence derived from protein synthetic patterns and in vitro translation experiments that a major qualitative shift in the mRNA population occurs in the 2-cell embryo.

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Additional Information:© 1987 The Company of Biologists Limited. Accepted 28 August 1987. We thank Sam Rose and Linda Western for sequencing the cDNA clones, and Gretchen De Nike, Alex Muller and Amir Reuveni for technical assistance. DNA sequence homology searches were carried out through the BIONET™ National Computer Resource for Molecular Biology whose funding is provided by NIH Grant No. RR-01685-03. This work was supported by the Medical Research Service of the Veterans Administration and by Public Health Service Research Grant HD-19691 from the National Institute of Child Health and Human Development.
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Veterans Administration Medical Research ServiceUNSPECIFIED
National Institute of Child Health and Human Development Public Health Service ResearchHD-19691
Subject Keywords:mouse embryo; mRNA; Bl and B2 repeats; cDNA clones
Issue or Number:4
Record Number:CaltechAUTHORS:20120626-093710455
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32086
Deposited On:26 Jun 2012 19:23
Last Modified:03 Oct 2019 03:57

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