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The Amino Terminus of the Yeast F_1-ATPase β-Subunit Precursor Functions as a Mitochondrial Import Signal

Emr, Scott D. and Vassarotti, Alessio and Garrett, Jinnie and Geller, Bruce L. and Takeda, Masaharu and Douglas, Michael G. (1986) The Amino Terminus of the Yeast F_1-ATPase β-Subunit Precursor Functions as a Mitochondrial Import Signal. Journal of Cell Biology, 102 (2). pp. 523-533. ISSN 0021-9525. PMCID PMC2114072. doi:10.1083/jcb.102.2.523.

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The ATP2 gene of Saccharomyces cerevisiae codes for the cytoplasmically synthesized beta-subunit protein of the mitochondrial F1-ATPase. To define the amino acid sequence determinants necessary for the in vivo targeting and import of this protein into mitochondria, we have constructed gene fusions between the ATP2 gene and either the Escherichia coli lacZ gene or the S. cerevisiae SUC2 gene (which codes for invertase). The ATP2-lacZ and ATP2-SUC2 gene fusions code for hybrid proteins that are efficiently targeted to yeast mitochondria in vivo. The mitochondrially associated hybrid proteins fractionate with the inner mitochondrial membrane and are resistant to proteinase digestion in the isolated organelle. Results obtained with the gene fusions and with targeting-defective ATP2 deletion mutants provide evidence that the amino-terminal 27 amino acids of the beta-subunit protein precursor are sufficient to direct both specific sorting of this protein to yeast mitochondria and its import into the organelle. Also, we have observed that certain of the mitochondrially associated Atp2-LacZ and Atp2-Suc2 hybrid proteins confer a novel respiration-defective phenotype to yeast cells.

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Additional Information:© 1986 Rockefeller University Press. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at, or updates thereof. Received for publication 24 June 1985, and in revised form 29. October 1985. We are grateful to Marjorie Britten, Mac Biggs, and Jane Goldsborough for expert technical assistance. The work was supported by an award from the Searle Scholars Program (to S. D. Emr) and by National Institutes of Health Research Grants GM 32703 (to S.D. Emr) and GM 26713 (to M. G. Douglas). B. L. Geller was supported by a Postdoctoral Fellowship from the Robert A. Welch Foundation (1-814) Houston, Texas, and J. Garrett was supported by an E. S. Gosney Postdoctoral Research Fellowship, California Institute of Technology.
Funding AgencyGrant Number
Searle Scholars ProgramUNSPECIFIED
NIHGM 32703
NIHGM 26713
Robert A. Welch FoundationUNSPECIFIED
Caltech E. S. Gosney Postdoctoral Research FellowshipUNSPECIFIED
Issue or Number:2
PubMed Central ID:PMC2114072
Record Number:CaltechAUTHORS:20120627-080630340
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Official Citation:The amino terminus of the yeast F1-ATPase beta-subunit precursor functions as a mitochondrial import signal. S D Emr, A Vassarotti, J Garrett, B L Geller, M Takeda, and M G Douglas 102:523-533. doi:10.1083/jcb.102.2.523
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32123
Deposited By: Ruth Sustaita
Deposited On:27 Jun 2012 15:30
Last Modified:09 Nov 2021 21:15

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