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Molecular weight and structural nonequivalence of the mature ɑ subunits of Torpedo californica acetylcholine receptor

Conti-Tronconi, Bianca M. and Hunkapiller, Michael W. and Raftery, Michael A. (1984) Molecular weight and structural nonequivalence of the mature ɑ subunits of Torpedo californica acetylcholine receptor. Proceedings of the National Academy of Sciences of the United States of America, 81 (9). pp. 2631-2634. ISSN 0027-8424. PMCID PMC345123. doi:10.1073/pnas.81.9.2631.

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A discrepancy of about 20% exists between the molecular weight of the ɑ subunit of Torpedo californica electroplax acetylcholine receptor as determined by gel electrophoresis of the mature protein (M_r 40,000 ± 2000) and by nucleotide sequence analysis of cDNA (M_r ≈z50,000). We demonstrate by amino acid sequence analysis that post-translational processing does not occur and that the mature subunit has a M_r of ≈50,000. The functional acetylcholine receptor contains two copies of this ɑ subunit in addition to one each of related β, y, and δ subunits. The binding sites for cholinergic ligands that are located on the a subunits have been shown to be nonequivalent. Amino acid sequence analysis of peptides obtained by proteolytic cleavage of the a subunit reveals that N-asparagine glycosylation at a single site (residue 141) occurs to a different extent in the two copies of this polypeptide in the mature protein and provides an explanation for nonequivalence of their binding sites.

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Additional Information:© 1984 National Academy of Sciences. Communicated by John D. Roberts, December 27, 1983. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:N-glycosylation, sequence analysis, peptide maps, affinity labeling, post-translational modification
Issue or Number:9
PubMed Central ID:PMC345123
Record Number:CaltechAUTHORS:20120702-070128826
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Official Citation:Molecular Weight and Structural Nonequivalence of the Mature α Subunits of Torpedo californica Acetylcholine Receptor(pp. 2631-2634) Bianca M. Conti-Tronconi, Michael W. Hunkapiller and Michael A. Raftery Stable URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32219
Deposited By: Ruth Sustaita
Deposited On:02 Jul 2012 17:18
Last Modified:09 Nov 2021 21:25

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