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Amino-Terminal Sequence of the Tn3 Transposase Protein

Ditto, Mary D. and Chou, Joany and Hunkapiller, Michael W. and Fennewald, Michael A. and Gerrard, Stephen P. and Hood, Leroy E. and Cohen, Stanley N. and Casadaban, Malcolm J, (1982) Amino-Terminal Sequence of the Tn3 Transposase Protein. Journal of Bacteriology, 149 (1). pp. 407-410. ISSN 0021-9193. PMCID PMC216644.

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The amino-terminal sequence of the Tn transposase protein was determined to be Pro-Val-Asp-Phe-Leu-Thr-Thr-Glu-Gln-Val-Glu-Ser.... This was determined both from an active transposase protein purified from a transposase overproducing mutant strain and from a hybrid transposase-β-galactosidase fusion protein. The amino acid sequence corresponded to the DNA sequence of the transposase gene beginning at an ATG initiation codon, as previously predicted from the analysis of transposase-β-galactosidase gene fusions.

Item Type:Article
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Hood, Leroy E.0000-0001-7158-3678
Additional Information:© 1982 American Society for Microbiology. Received 20 July 1981; Accepted 16 September 1981. We thank Nicholas Cozzarelli for advice. This work was supported by Public Health Service grants GM 29067 and GM 06965 from the National Institute of General Medical Sciences to M.C. and L.H., respectively, and by National Science Foundation grant PCM 80-05599 to L.H. M.F. was supported by a fellowship from the Damon Runyon-Walter Winchell Cancer Fund (DRG-260-FT).
Funding AgencyGrant Number
NIHGM 29067
NIHGM 06965
NSFPCM 80-05599
Damon Runyon-Walter Winchell Cancer FundDRG-260-FT
Issue or Number:1
PubMed Central ID:PMC216644
Record Number:CaltechAUTHORS:20120716-143303838
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Official Citation:Amino-terminal sequence of the Tn3 transposase protein. M D Ditto, J Chou, M W Hunkapiller, M A Fennewald, S P Gerrard, L E Hood, S N Cohen, and M J Casadaban J. Bacteriol. January 1982 149:407-410
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32484
Deposited On:16 Jul 2012 21:46
Last Modified:03 Oct 2019 04:01

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