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Purification and Properties of a Host Cell Protein Required for Poliovirus Replication in Vitro

Baron, Margaret H. and Baltimore, David (1982) Purification and Properties of a Host Cell Protein Required for Poliovirus Replication in Vitro. Journal of Biological Chemistry, 257 (20). pp. 12351-12358. ISSN 0021-9258.

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A host cell protein required for poliovirus RNA-dependent RNA replicase activity in vitro has been purified several thousand-fold from an uninfected HeLa cell postmitochondrial supernatant. A single protein of apparent Mr = approximately 67,000 daltons and pI 6.3 is associated with this "host factor" activity. Poly(U)-Sepharose chromatography of the template-dependent replicase isolated from poliovirus-infected cells results in the complete loss of replicase activity if a salt gradient is used to develop the column. Host factor elutes early in the salt gradient and restores replicase activity to protein fractions eluted later in the gradient. The host factor, estimated to be present at 50,000-100,000 copies/cell, interacts physically with replicase.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1982 American Society for Biochemistry and Molecular Biology. Received for publication, February 2, 1982. This research was supported by grants from the National Institute of Allergy and Infectious Diseases and from the National Cancer Institute. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Predoctoral scholar of the Insurance Medical Scientist Scholarship Fund and fellow of the Danforth Foundation. American Cancer Society Professor of Microbiology.
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National Institute of Allergy and Infectious DiseasesUNSPECIFIED
National Cancer InstituteUNSPECIFIED
Danforth FoundationUNSPECIFIED
Issue or Number:20
Record Number:CaltechAUTHORS:20120717-091213541
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Official Citation:Purification and properties of a host cell protein required for poliovirus replication in vitro. M H Baron and D Baltimore J. Biol. Chem. 1982 257: 12351-12358.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32500
Deposited By: Ruth Sustaita
Deposited On:17 Jul 2012 18:01
Last Modified:03 Oct 2019 04:01

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