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Synthesis of Murine Leukemia Virus Plus Strong Stop DNA Initiates at a Unique Site

Mitra, Sudha W. and Chow, Marie and Champoux, James and Baltimore, David (1982) Synthesis of Murine Leukemia Virus Plus Strong Stop DNA Initiates at a Unique Site. Journal of Biological Chemistry, 257 (11). pp. 5983-5986. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:20120717-133113420

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Abstract

The 5'-terminal segment of plus strong stop DNA has been isolated from an endogenous virion reverse transcription reaction and characterized. The plus strong stop DNA is shown to initiate with the sequence 5'AATGAAAGA.... The 5'-terminal nucleotide is a deoxyribonucleotide. No residual protein or RNA moiety was found attached to the plus strong stop DNA. Thus, the mechanism which primes plus strand synthesis is still unclear. No evidence for multiple initiation sites or heterogeneous starts is observed. This data indicates that the initiation of plus strong stop DNA synthesis by the virion RNA-dependent DNA polymerase is a precise event which occurs at a unique site on the retrovirus genome.


Item Type:Article
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http://www.jbc.org/content/257/11/5983.abstractPublisherUNSPECIFIED
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 1982 by American Society for Biochemistry and Molecular Biology. Received for publication, February 8, 1982. This work was supported by grants from the National Cancer Institute. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Postdoctoral fellow of the American Cancer Society, Massachusetts Division. Present address, Department of Biochemical Genetics, Merck Sharp and Dohme Research Laboratories, Rahway, NJ 07065. Aided by American Cancer Society Grant PF-1857. Supported by a Guggenheim Foundation Fellowship. Present address, Department of Microbiology, University of Washington, School of Medicine, Seattle, WA 98195. American Cancer Society Research Professor.
Funders:
Funding AgencyGrant Number
National Cancer InstituteUNSPECIFIED
American Cancer SocietyPF-1857
Guggenheim Foundation FellowshipUNSPECIFIED
Record Number:CaltechAUTHORS:20120717-133113420
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120717-133113420
Official Citation:Synthesis of murine leukemia virus plus strong stop DNA initiates at a unique site. S W Mitra, M Chow, J Champoux, and D Baltimore J. Biol. Chem. 1982 257: 5983-6.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32525
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:17 Jul 2012 21:21
Last Modified:31 Oct 2017 18:08

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