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Multiple differences between the nucleic acid sequences of the IgG2a^a and IgG2a^b alleles of the mouse

Schreier, Peter H. and Bothwell, Alfred L. M. and Mueller-Hill, Benno and Baltimore, David (1981) Multiple differences between the nucleic acid sequences of the IgG2a^a and IgG2a^b alleles of the mouse. Proceedings of the National Academy of Sciences of the United States of America, 78 (7). pp. 4495-4499. ISSN 0027-8424.

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To compare the structure of IgG2a alleles we have determined the complete DNA sequence of the constant region, coding sequence, and 3' untranslated region of a cDNA clone, pAB gamma 2a-1, which was derived from the C57BL/6 mouse strain (b allotype). This sequence was compared with the corresponding IgG2a DNA sequence of BALB/c origin (a allotype). The DNA sequences showed 10% differences, and the deduced protein sequences differed by about 15%. These differences were not evenly distributed: most differences were in the hinge region, the CH3 domain and the 3' untranslated region. It is evident that many alterations in the IgG2a alleles have occurred since the a and b haplotypes were separated--some of these changes were point mutations but some appear to have resulted from gene conversion of the IgG2a^b allele by the IgG2b^b allele.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1981 National Academy of Sciences. Contributed by David Baltimore, April 3, 1981. We thank Drs. M. A. Davies, T. Imanishi-Kari, and K. Rajewsky for stimulating and encouraging discussions, S. Quester for excellent technical assistance, and W. Huelsenbeck for help with some of the experiments. This work was supported by the Deutsche Forschungsgemeinschaft through SFB 74 to B.M.-H. and by grants from the American Cancer Society and the National Cancer Institute to D.B.; A.B. is a Postdoctoral Fellow of the Medical Foundation; D.B. is an American Cancer Society Professor of Microbiology. The publication costs ofthis article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.
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Deutsche Forschungsgemeinschaft (DFG)SFB 74
American Cancer SocietyUNSPECIFIED
National Cancer InstituteUNSPECIFIED
Medical Foundation Postdoctoral FellowshipUNSPECIFIED
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ID Code:32548
Deposited By: Jason Perez
Deposited On:18 Jul 2012 17:47
Last Modified:31 Oct 2017 18:04

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