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Purification of a factor that restores translation of vesicular stomatitis virus mRNA in extracts from poliovirus-infected HeLa cells

Trachsel, Hans and Sonenberg, Nahum and Shatkin, Aaron J. and Rose, John K. and Leong, Kahan and Bergmann, John E. and Gordon, Julian and Baltimore, David (1980) Purification of a factor that restores translation of vesicular stomatitis virus mRNA in extracts from poliovirus-infected HeLa cells. Proceedings of the National Academy of Sciences of the United States of America, 77 (2). pp. 770-774. ISSN 0027-8424. doi:10.1073/pnas.77.2.770.

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It was previously shown that the poliovirus-induced inhibition of translation of capped mRNAs can be reversed by a protein found in preparations of the eukaryotic initiation factor eIF-4B [Rose, J. K., Trachsel, H., Leong, K. & Baltimore, D. (1978) Proc. Natl. Acad. Sci. USA 75, 2732--2736]. This "restoring factor" has now been purified from a high-salt wash of rabbit reticulocyte ribosomes by taking advantage of its tight association with factor eIF-3 at low salt concentrations. It did not copurify with the major M_r 80,000 polypeptide of eIF-4B preparations but did copurify with a M_r 24,000 polypeptide previously shown to bind to the cap structures of mRNAs [Sonenberg, N., Rupprecht, K. M., Hecht, S. M. & Shatkin, A. J. (1979) Proc. Natl. Acad. Sci. USA 76, 4345--4349]. Both the electrophoretic mobility and the tryptic peptide pattern of the restoring factor were indistinguishable from those of the cap-binding protein, and the restoring factor could be crosslinked to the 5'-terminal cap on mRNA. Thus, is appears that poliovirus inhibits cellular protein synthesis by inactivation of some crucial property of the cap-binding protein.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1980 National Academy of Sciences. Contributed by David Baltimore, October 22, 1979. The portion of this work that was done at Massachusetts Institute of Technology was supported by Grants AI-14566 (to J.K.R.) and Al-08388 (to D.B.) from the National Institute of Allergy and Infectious Diseases and Grant CA-14051 from the National Cancer Institute (core grant to S. E. Luria). D.B. is an American Cancer Society Professor of Microbiology. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
National Institute of Allergy and Infectious DiseasesAI-14566
National Institute of Allergy and Infectious DiseasesAl-08388
National Cancer InstituteCA-14051
Subject Keywords:translational control; eukaryotic initiation factor 4B; cap-binding protein; translation in vitro
Issue or Number:2
Record Number:CaltechAUTHORS:20120718-141059759
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32559
Deposited On:19 Jul 2012 14:41
Last Modified:09 Nov 2021 21:28

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