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Direct Atomic Force Microscopy Observation of DNA Tile Crystal Growth at the Single-Molecule Level

Evans, Constantine G. and Hariadi, Rizal F. and Winfree, Erik (2012) Direct Atomic Force Microscopy Observation of DNA Tile Crystal Growth at the Single-Molecule Level. Journal of the American Chemical Society, 134 (25). pp. 10485-10492. ISSN 0002-7863. doi:10.1021/ja301026z. https://resolver.caltech.edu/CaltechAUTHORS:20120720-132124836

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Abstract

While the theoretical implications of models of DNA tile self-assembly have been extensively researched and such models have been used to design DNA tile systems for use in experiments, there has been little research testing the fundamental assumptions of those models. In this paper, we use direct observation of individual tile attachments and detachments of two DNA tile systems on a mica surface imaged with an atomic force microscope (AFM) to compile statistics of tile attachments and detachments. We show that these statistics fit the widely used kinetic Tile Assembly Model and demonstrate AFM movies as a viable technique for directly investigating DNA tile systems during growth rather than after assembly.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja301026zDOIUNSPECIFIED
http://pubs.acs.org/doi/abs/10.1021/ja301026zPublisherUNSPECIFIED
ORCID:
AuthorORCID
Evans, Constantine G.0000-0002-7053-1670
Winfree, Erik0000-0002-5899-7523
Additional Information:© 2012 American Chemical Society. Published In Issue June 27, 2012; Article ASAP June 13, 2012; Received: February 13, 2012. We thank Christina Wright for prototype software for analyzing AFM movies, and Shaun Lee for discussions regarding AFM movies of DNA tiles. We acknowledge National Science Foundation (NSF) Awards 0832824 (“The Molecular Programming Project”) and 0622254.
Funders:
Funding AgencyGrant Number
NSF0832824
NSF0622254
Issue or Number:25
DOI:10.1021/ja301026z
Record Number:CaltechAUTHORS:20120720-132124836
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120720-132124836
Official Citation:Direct Atomic Force Microscopy Observation of DNA Tile Crystal Growth at the Single-Molecule Level Constantine G. Evans, Rizal F. Hariadi, and Erik Winfree Journal of the American Chemical Society 2012 134 (25), 10485-10492
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32617
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:23 Jul 2012 16:31
Last Modified:09 Nov 2021 21:28

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