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Primer Requirement and Template Specificity of the DNA Polymerase of RNA Tumor Viruses

Baltimore, David and Smoler, Donna (1971) Primer Requirement and Template Specificity of the DNA Polymerase of RNA Tumor Viruses. Proceedings of the National Academy of Sciences of the United States of America, 68 (7). pp. 1507-1511. ISSN 0027-8424. doi:10.1073/pnas.68.7.1507.

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Polyribonucleotides will act as efficient templates for the DNA polymerases found in the virions of avian myeloblastosis virus and mouse leukemia virus if a short complementary oligodeoxyribonucleotide primer is added. Synthesis of the complementary polydeoxyribonucleotide continues until an amount of polymer equal to the amount of initial template has been produced. The two viruses show slightly different specificities toward the four homoribopolymers. Polydeoxyribonucleotides are generally much poorer templates than the homologous polyribonucleotides, in most cases yielding no detectable synthesis. The DNA polymerase of RNA tumor viruses, therefore, have the same requirements for activity as do other DNA polymerases, except that they prefer polyribonucleotides over polydeoxyribonucleotides as templates.

Item Type:Article
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Baltimore, David0000-0001-8723-8190
Additional Information:© 1971 National Academy of Sciences. Communicated by Alexander Rich, April 26, 1971. We thank Drs. F. Bollum, O. Friedman, J. Huberman, and A. Kornberg for providing materials used in this study and for advice about their properties. We thank Dr. I. Verma for his help during this work. We have been supported by grants no. AI-08388 from the USPHS and grant no. E-512 from the American Cancer Society. D. B. is a Faculty Research Awardee of the American Cancer Society.
Funding AgencyGrant Number
Public Health ServiceAI-08388
American Cancer SocietyE-512
Subject Keywords:avian myeloblastosis virus; mouse leukemia virus; E. coli DNA polymerase; homopolynucleotides; oligonucleotides
Issue or Number:7
Record Number:CaltechAUTHORS:20120724-132033639
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32681
Deposited On:24 Jul 2012 23:14
Last Modified:09 Nov 2021 21:29

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